Recently, we had the opportunity to get involved with a cutting-edge tool for genetic research developed by the company GNAcode from Denmark. They have designed and produced the MiniCube PCR, an asynchronous thermo-cycler. This 16 well PCR machine, quite different from other thermo-cyclers, allows multiple different DNA amplification protocols or incubations to be carried out in parallel, time-shifted parallel or serial. Potentially opening for an unseen complexity in experimental design but also giving the user a flexibility in experimentation not possible with other devices.
SCIPY FOR MINICUBE BY OUR COMPANY
We come to our role in this whole process, namely: the Open-Source Scientific Python software. The machine is equipped with an Open-Source Application interface to allow the users to create their own programs that transfer protocols to the machine for execution, monitor status, and extract and analyze thermal data related to the experiments. We took upon us the task to create a Jupyter Notebook environment for communication with the machine and then setup an example experiment and then assign it to the machine for execution. Basic work – but the frame for lots of new Jupyter Notebook applications creating advanced protocols as e.g. ”precision gradient step” experiments for primer optimization, ”calorimetric analysis of evaporation”, ”thermal analysis of reactions” to name a few.
The development is Open-Source and available and we have really appreciated the opportunity to be the first-external-developers on this platform. Scientists can now connect with Jupyter Notebook to the MiniCube and create really exciting new applications for genetic experimentation and analysis. Naturally, we are available for consultancy or service if required. At the same time, we would like to say it is a real pleasure to work with the engineers and scientists of the GNAcode company.
We really appreciate the Open-Source opportunity allowing external professionals to create biotech software on such an advanced instrumentation system for genetic research.
We are honored to announce that we have become an official certified Open-Source business developer for the Minicube Platform from GNAcode.
THE POLYMERASE CHAIN REACTION
The polymerase chain reaction abbreviated PCR is a technique where a fragment of DNA is copied using a thermo-stable bacterial enzyme called a polymerase. This technique (see notes below) using cooling and heating cycles to separate the DNA and creating a new set of copies of the fragment can be repeated - till we receive a high quantity of DNA allowing analysis of the fragment with other techniques.
ASYNCHRONOUS PCR BY THE MINICUBE
The Minicube PCR machine can as an example run a PCR protocol on e.g. 4 wells, then later start an incubation at e.g. 50 °C at two other wells running a reverse transcription where messenger RNA is converted to cDNA and then at some point later start a second incubation at one well at 40 °C, and a co-worker can insert a row of 8 tubes an initiate a second PCR reaction. To fully explain the asynchronous function of the MiniCube PCR it can run 16 different protocols at the same time meaning 16 different PCR protocols with different temperature stages. That is possible due to the system of single good control coupled with the thermal zero-crosstalk features of the system allowing perfectly controlled thermal reactions to co-exist within just a few millimeters from each other.
The device is ideal for optimization of protocols, preparation for sequencing, cloning, multiple parallel incubations. In normal mode, working on standard equipment, these research had to be conducted in series, now everything can be run in parallel.
35 cycles in 21 minutes and 40 s are possible and all because of the peak ramping speed of 9°C/sec. Each of the MiniCubes 16 wells is NIST temperature calibrated and during runtime, the temperature is adjusted 100 times per second by a microprocessor.
NOVEL POSSIBILITIES IN PCR
The MiniCube PCR is a real breakthrough for thermo-cycling – not due to its high flexibility which is a “very nice feature”, but due to the very high degree of both accuracy and precision of the device coupled to the control-feedback ensuring perfectly controlled PCR reactions which not really has been achieved other than in lab-on-chip systems and due to the features the control-feedback enables. According to the inventors of the device, it can amoung many things be utilized to monitor the evaporation of liquid from the test tubes since each well effectively is a precision calorimeter which can measure the amount of mass in the tube.
MINICUBE APP FROM APPLE APP STORE
What else? - you will ask, and I would say: MiniCube App. The iPad App (search for “GNAPCR” in Apple App store) gives full control over the process, planning of experiments can be done in the office and execution later in the lab. The iPad can control multiple machines at the same time, and other users can equally at the same time have parallel access to the MiniCube or MiniCubes – making it a great tool in a busy community lab.
Notes: Polymerase Chain Reaction
The polymerase chain reaction abbreviated PCR is a technique where DNA is copied using a thermo-stable bacterial enzyme called a polymerase which can withstand boiling. The DNA consisting of two double strands is initially heated in which the thermal energy causes the two double strands to separate – in our cells this occurs at 37 °C using more complex enzymes but the PCR technique uses simply the heat to separate the DNA and the bacterial enzyme can withstand it because it is isolated from bacteria normally living in hot springs where life exists at boiling point. Now the DNA is separated and the machine cools the test-tubes down.
The reaction soup in the test-tubes contains smaller pieces of DNA called primers, typically 20 bases long, which can create a binding to the separated DNA strands at an elevated temperature typically between 50-65 °. It also among other things contains nucleotides which are the building blocks of DNA. When the temperature reaches the “binding temperature” also called “annealing temperature” of the primers they will bind to the separated DNA strands and the polymerase can use this complex of “primer” and DNA strand to initiate elongation or copying of the DNA strand by incorporation of new nucleotides from the point where the primer ends. Now, two new strands of DNA will be formed.
The PCR technique uses two different primers and they can be designed so that one primer binds to one DNA strand and the other primer binds to the opposite DNA strand and a double-stranded DNA fragment is formed which is the length of the distance between the two primers including themselves. If the reaction is heated again the newly formed double-stranded DNA will be melted again forming a double number of strands equal to 2x where x is the number of times the heating and cooling cycle is repeated. Typically, PCR reactions are carried out in a range of heating and cooling cycles between 30-40 times. This gives a copy number of 240 equal to 1099511627776 number of DNA strands made out of a starting material of just 1 DNA copy.
In reality, this number is never so high because there is a limitation to how many copies can be built due to how many nucleotides and primers that initially are in the reaction mixture.
2017-12-03 19:45:03 +0100
Buy Medical Marijuana Online | Buy Weed Online | Buy Ganja Hash Onlinehttp://www.gonaturalpharmacy.com/ Title: Buy Medical Marijuana Online | Buy Weed Online | Buy Ganja Hash Online http://www.gonaturalpharmacy.com/ +1-518-314-9293 Buy xanax online , buy painkillers online http://gonaturalpharemacy.skyrock.com/ Buy Medical Marijuana Online at GoNaturalPharmacy.com worldwide online store. Buy Weed Ganja Hash online. Get high quality Medical Marijuana at best price. https://www.youtube.com/watch?v=j1HHqoW6nzE https://vimeo.com/244714589 https://ordercannabisonline.wordpress.com http://www.gonaturalpharmacy.com/ https://buybudse.blogspot.co.uk/ mail order weed online | buy real weed online | buy illegal weed online | buy real weed on cheap | online dispensary shipping| weed store usa | buy legal weed online | buy skunk online uk | kush for sale | 420 mail order | buy real has online | best only dispensary | is it legal to buy weed online | online dispensary shipping | dispensaries that ship out of state | kush for sale near me | legal kush for sale | kush for sale online | buy OXYCOTIN online |buy skunk online uk | online dispensary shipping is it legal to buy edibles online |420 mail order medicine man online dispensary legit |dispensaries that | ship out of state | og kush for sale online Order PERCOCET Online | buy OXYCOTIN online | | kush for sale |buy skunk online uk | online dispensary shipping | 420 mail order | is it legal to buy |edibles online | dispensaries that ship out of state | medicine man online dispensary legit | buy charas online india | | online dispensary shipping | 420 mail order | is it legal to buy edibles online | medicine man online dispensary legit | buy skunk online uk | dispensaries that ship out of state | kush for sale | | recreational dispensaries near me | medical dispensary near me | recreational dispensaries | near me open late | clones bend oregon | weedmap | bloom well bend, or | weedmaps deals | dispensaries near me open now | | buy illegal weed online | mail order weed online | buy recreational weed online | buying weed online reddit | buy real weed online | online dispensary shipping | buy legal weed online | weed store usa | | order oxycodone 30mg online | canadian pharmacy oxycodone | buy oxycodone uk online | anyone know of a legit online pharmacy for oxycodone | buy oxycontin online legally | order oxycodone online reviews | oxycodone online cod | cheap oxycodone online overnight delivery | | 30 mg adderall street price 2017 | 20 mg adderall price per pill 2017 | street value of adderall 10mg | 20 mg adderall price per pill 2016 | adderall xr 30 mg high | 20 mg adderall price per pill 2015 | adderall 10mg street price 2015 | adderall 30 mg cost | | buy painkillers online cheap | buy narcotics online legally | buy strong painkillers online | buy pain meds online overnight | buy prescription pills online | chemdrugstore reviews | buy pain meds online with credit card | buy pain pills online reviews | | mail order weed online | buy illegal weed online | buy real weed online | online dispensary shipping | buy recreational weed online | buying weed online reddit | buy legal weed online | is it legal to buy edibles online | | buy pain meds online legally | how to get prescription | drugs without a doctor | prescription drugs online without can you buy prescription drugs online without | buy prescription drugs online overseas | buy prescription drugs online cheap | buying prescription drugs from canada | mail order prescription drugs from canada | | online dispensary shipping | medicine man online dispensary legit | dispensaries that ship out of state | 420 mail order | kush for sale is it legal to buy edibles online | buy skunk online uk | online dispensary edibles | buy cheap weed online usa | buy grams of weed online | buy illegal weed online | mail order weed online | weed for sale craigslist | buy recreational weed online | buy legal weed online | online dispensary shipping |
2017-12-03 17:39:31 +0100
Testimony of financial breakthrough from GOD through the help of Funding Circle Loan INC. (email@example.com OR Call/Text +14067326622)... Hi I'am Evelyn Russell resident at 808 NE 19th St Oklahoma City, I am a single mother blessed with 2 daughters. For a while now I have been searching for a genuine loan lender who could help me with a loan as I no longer have a job, all I got were hoodlums who made me trust them and at the end they took my money without giving me any loan, my hope was lost, I got confused and frustrated, it became difficult for my family to feed with a good meal, I never wanted to have anything to do with any loan lending companies on the internet again. Not until I met a Godsent loan lender that changed my life and that of my family Through the help of a fellowship member "a lender with the fear of God in him Mr JASON RAYMOND, he was the man God sent to elevate my family from suffering. At first I thought it wasn’t going to be possible due to my previous experience until I received my loan worth $135,000.00 USD in less then 24hours. So my advise to anyone out there genuinely in need of a loan is to contact Funding Circle Loan INC through this official email:- firstname.lastname@example.org OR Call/Text +1(406)732-6622 and be financial lifted.