- Central European Biotechnology Portal
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Expression and purification of novel proteins

Czech Republic
  • gene cloning from original organism
  • gene insertion into expression plasmid
  • plasmid transformation to host organism
  • recombinant over-expression
  • selection of host organism
  • optimization of over-expression
  • semi-continuous cultivation
  • fermentation
  • cell disruption (sonication, homogenisation)
  • basic protein separation (membrane filtration, centrifugation, salt precipitation)
  • protein purification and optimization analysis of protein purity
  • analysis of post-translation modifications (mass spectrometry)

Enantis, s.r.o.

Palackeho trida 1802/129
612 00 Brno Czech Republic