be visible on the BioBusiness market ISSN 2300-0228
Expression and purification of novel proteins
- gene cloning from original organism
- gene insertion into expression plasmid
- plasmid transformation to host organism
- recombinant over-expression
- selection of host organism
- optimization of over-expression
- semi-continuous cultivation
- fermentation
- cell disruption (sonication, homogenisation)
- basic protein separation (membrane filtration, centrifugation, salt precipitation)
- protein purification and optimization analysis of protein purity
- analysis of post-translation modifications (mass spectrometry)
