The Quantitative PCR Core Facility is situated at the Institute of Biotechnology AS CR, v.v.i. as a part of Laboratory of Gene Expression. The Quantitative PCR Core Facility provides high-throughput gene expression analyses using real-time quantitative PCR with a high-throughput instrument BioMark^TM from Fludigm. The BioMark arrays enable qPCR use for both validation and discovery studies by radically increasing sample and assay throughput. The PCR core facility has also the following major equipment available: CFX96 and CFX384 thermocyclers from Bio-Rad, StepOne Real-Time PCR System and 7500 Fast Real-Time PCR System from Applied Biosystems, LightCycler480 II from Roche and a rotor based Rotor Gene 6000 from Qiagen. Samples can be processed and assay plates assembled utilizing a robotic liquid handling workstation Eppendorf R epMotion T 5070 Workstation. Take advantage of our knowledge and let us perform your analyses with high quality and previously unachievable throughput.

The offered services:

-  High throughput gene expression analysis performed at BioMark^TM System from Fludigm with 48.48 Gene Expression Dynamic Array, which is producing 2,304 datapoints. You can analyse 48 samples with 48 gene assays simultaneously.

-  High throughput gene expression analysis performed at BioMark^TM System from Fludigm with 96.96 Gene Expression Dynamic Array, which is producing 9,216 datapoints. You can analyse 96 samples with 96 gene assays simultaneously.

-  Gene specific preamplification of your sample.

-  Regular throughput gene expression analysis performed by qPCR instrument of your choice.