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Modern Mass-Spec Core Facility

Modern Mass-Spec Core Facility

Publish date: 2015-02-22

The new laboratory of mass spectrometry is one of the most important achievements of the project MOBI4Health implemented in the Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Poland. Modern equipment allows to perform virtually any type of analysis, from the identification of small molecule compounds and organic substances, through the analysis of the nucleic acid sequence, to proteome profiling.

Tags: MOBI4Health , mass spectrometry , proteomics

Center of Molecular Biotechnology for Healthy Life - Biotech solutions bringing health to living organisms and environment supported by mass spec-focused research platform, acronym MOBI4Health, is the winner project of REGPOT competition, funded by the European Commission under the 7th Framework Programme of the EU. The project valued at over 5 million euro is carried out in cooperation with the ten European research centers. The aim is to increase the potential of IFB UG&MUG in the field of molecular biotechnology.

One of the project work packages, thus the development of research infrastructure, has been accomplished through the creation of well-equipped mass spectrometry core facility. The set of apparatus available in the laboratory is unique on the national scale. Core facility includes Laboratory of Genetic Analysis and Laboratory of Mass Spectrometry, equipped with modern devices. MassARRAY Nanodispenser RS1000 and MassARRAY Analyzer 4 are an integrated platform utilizing MALDI-TOF technology for analyzing nucleic acids, including genotyping, quantitative analysis of transcripts and DNA methylation. MALDI-TOF/TOF 5800 enables the identification and quantification of proteins, analysis of biomarkers and spatial MALDI imaging. QTRAP 6500 with SelexIon utilizing LC/MS/MS technology allows for the effective separation, detection and quantification of compounds in complex samples. TripleTOF 5600+, also using high resolution LC/MS/MS technology, allows for qualitative and quantitative analysis in proteomics, metabolomics and lipidomics, using SWATH mode of analysis.

Within the project, a series of trainings and lectures are organized, showing the possibilities of  apparatus usage in various fields of research. To date, two workshops have been organized, regarding the use of mass spectrometry in proteomic and genomic analysis and also molecular diagnostics of diseases in humans, animals and plants. In 2015, there are two more upcoming meetings: Innovations in environmental friendly productions of lipids for industry (March 17-19, Gdansk) and Innovations in discovery of potential new pharmaceuticals (July 7-9, Gdansk). The workshops will be divided into the open lecture part and the practical course available to employees of IFB UG&MUG. Events agenda will be released soon on the project website.

MOBI4Health Center gives the opportunity of a comprehensive research grants implementation and scientific cooperation. - We expect our research topics and ideas to reach the widest possible group of researchers. Our goal is to change the public conviction that in Polish science nothing is happening, and public money is being wasted - says project coordinator Professor Krzysztof Bielawski.

One of the research projects implemented in the Intercollegiate Faculty of Biotechnology considers HBV drug resistance in the context of genetic polymorphism of the virus. The study uses the capabilities of the MassARRAY platform (Rybicka 2013; Stalke 2014). For more information visit the project website.

Monika Kossakowska-Zwierucho

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