- Central European Biotechnology Portal
be visible on the BioBusiness market                  ISSN 2300-0228
Mutations in a cell line stops stem-cell trial to cure blindness

Mutations in a cell line stops stem-cell trial to cure blindness

Publish date: 2015-08-12

Induced pluripotent stem cells (iPSC) turned into specialized retinal cells were meant to cure the damage caused by age-related macular degeneration leading to blindness. The trial was halted due to mutations identified in cells of a second patient.

Tags: clinical trials , stem cells , mutations , macular degeneration , blindness , iPSC

iPSC of non-embryonic origin are derived from mature, differentiated cells reprogrammed to a state of pluripotency, which then can be again differentiated to virtually any type of specialized cells. Such cells was developed by the team of clinical researcher Dr. Masayo Takahashi in Japan. Human retinal epithelium sheet were derived from patient’s own skin cells and would serve as an autogenic transplant.

After a successful transplantation in a first patient in 2014, a trial was recently suspended before transplanting cells into a second patient’s eye. The reason is that their iPSC did not pass the genomic validation step, revealing six mutations in a cell line – three gene deletions and three single nucleotide mutation, one within an oncogene. Mutations could exist in original skin cells at undetectable level or could be acquired during iPSC reprogramming procedure.

Since the risk of carcinogenesis related to oncogene mutation was assessed low, and stem cells specialists presented different view of an overall danger related to the therapy, a trail was suspended for a time being. An additional reason for halting is a change in Japan’s regulation concerning types of institution can run stem-cell trials. After accommodating these changes, the team would introduce attempts to resume a research, thus an identification of the source of mutations, and on the other hand, testing mutation-free skin cells from younger donors for an allogeneic transplantation.

Source: New Scientist

Monika Kossakowska-Zwierucho


Add comment
2016-04-03 03:58:39 +0200
My name is Jessica Luis, and I base in USA...My life is back!!! After 1 years of Broken marriage, my husband left me with two kids . I felt like my life was about to end i almost committed suicide, i was emotionally down for a very long time. Thanks to a spell caster called Dr Mohammed, which i met online. On one faithful day, as I was browsing through the internet,I came across allot of testimonies about this particular spell caster. Some people testified that he brought their Ex lover back, some testified that he restores womb,cure cancer,and other sickness, some testified that he can cast a spell to stop divorce and so on. i also come across one particular testimony,it was about a woman called Sonia,she testified about how he brought back her Ex lover in less than 2 days, and at the end of her testimony she dropped Dr Mohammed's e-mail address. After reading all these,I decided to give it a try. I contacted him via email and explained my problem to him. In just 48hours, my husband came back to me. We solved our issues, and we are even happier than before Dr Mohammed, is really a gifted man and i will not stop publishing him because he is a wonderful man... If you have a problem and you are looking for a real and genuine spell caster to solve all your problems for you. Try High anytime, he might be the answer to your problems. Here's his contact: or call him +2348134493948
2016-04-03 02:17:04 +0200
'After being in relationship with Wilson for seven years,he broke up with me, I did everything possible to bring him back but all was in vain, I wanted him back so much because of the love I have for him, I begged him with everything, I made promises but he refused. I explained my problem to someone online and she suggested that I should contact a spell caster that could help me cast a spell to bring him back but I am the type that don't believed in spell, I had no choice than to try it, I meant a spell caster called Dr Zuma zuk and I email him, and he told me there was no problem that everything will be okay before three days, that my ex will return to me before three days, he cast the spell and surprisingly in the second day, it was around 4pm. My ex called me, I was so surprised, I answered the call and all he said was that he was so sorry for everything that happened, that he wanted me to return to him, that he loves me so much. I was so happy and went to him, that was how we started living together happily again. Since then, I have made promise that anybody I know that have a relationship problem, I would be of help to such person by referring him or her to the only real and powerful spell caster who helped me with my own problem and who is different from all the fake ones out there. Anybody could need the help of the spell caster, his email: or call him +2349055637784 you can email him if you need his assistance in your relationship or anything. CONTACT HIM NOW FOR SOLUTION TO ALL YOUR PROBLEMS'
See also
On 23rd February Immunotech Laboratory announced $2M funding approval from its associate Bulgarian Immunotech P... Read more
Tags: bulgaria , medicine , pharmaceutical , clinical trials
Aurigon Life Science is a full service contract research organisation offering optimized and tailor-made soluti... Read more
Tags: hungary , germany , CRO , clinical trials ,
Almost everyone has heard about cloning sheep Dolly. This week, the team from Oregon Health & Science Universit... Read more
Tags: stem cells , cloning , genetic engineering