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Rapid detection of Ebola virus

Rapid detection of Ebola virus

Publish date: 2015-10-19

The Ebola virus can be deadly. Depending on the virus strain, between 25 and 90 percent of those infected with the disease die. The virus is passed on through direct contact with the blood or body fluids of sick or already dead people, as well as infected wild animals. That is why it is vital to know as quickly as possible whether an individual with suspicious symptoms such as fever, headache, muscle aches, nausea or internal or external bleeding is infected.

Tags: diagnostics , ebola , RT-PCR

The Ebola Virus (EBOV) Real-Time RT PCR (Polymerase Chain Reaction) Kit has been presented at BIOTECHNICA 2015, by MoBiTec company. It can carry out rapid, targeted and sensitive testing almost anywhere worldwide when used with mobile laboratory equipment. The kit is currently being used in various countries in Europe, Asia, America and Africa.

Liferiver’s Ebola Virus Real Time RT-PCR Kit has been recently approved as eligible for WHO procurement. After conducting quality, safety and performance evaluation, the approval was issued. Interestingly, there are only three Ebola virus IVD reagents worldwide with such approval. Compared to the first PCR test approved by WHO in late 2014, which is also the benchmark for existing Ebola diagnostics, Liferiver™ is 10 times more sensitive based on the performance evaluation conducted by WHO.

The test is based on real-time RT-PCR technology, utilizing reverse transcriptase (RT) reaction to convert RNA into complementary DNA (cDNA), polymerase chain reaction (PCR) for the amplification of specific target sequences and target specific probes for the detection of the amplified DNA. The probes are labelled with fluorescent reporter and quencher dyes. Probes specific for Ebola virus RNA are labelled with the fluorophore FAM.

The probe specific for the target of the Internal Control (IC) is labelled with the fluorophore VIC. The test consists of three processes:

  1. Reverse transcription of target RNA to cDNA
  2. PCR amplification of target cDNA and Internal Control
  3. Simultaneous detection of PCR amplicons by fluorescent dye labelled probes

 

 

Aleksandra Kowalczyk

References:

http://www.who.int/diagnostics_laboratory/procurement/150427_liferiver_china_public_report.pdf

http://www.mobitec.com/cms/news/index.html

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