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Researchers from Olsztyn are working on Alzheimer's early detection tests

Researchers from Olsztyn are working on Alzheimer's early detection tests

Publish date: 2015-03-10

An effort to elaborate tests enabling early detection of Alzheimer’s disease is currently made in numerous research institutes all over the world. One of them is the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences. In an interview for cebiotech.com, Professor Hanna Radecka, a leader of research group working on early diagnosis of Alzheimer’s disease, presents the basis of detection tests and future possibilities for prophylaxis and more efficient treatment.

Tags: Alzheimer's disease , amyloid , olsztyn , diagnosis , prevention , sensor , Hanna Radecka

The method of early diagnosis of Alzheimer’s disease that you are working on is based on the detection of specific markers in the patient’s blood. What is the basis of the test and what markers apart from β-amyloid can be useful?

Professor Hanna Radecka: Currently, the area of Alzheimer’s disease (AD) detection is being intensively developed. In our studies we are not focused on searching for novel markers. Instead, we cooperate with researchers from Institute of Biochemistry and Biophysics of Polish Academy of Sciences in Warsaw  that are specialized in the investigation of potent markers useful in terms of AD diagnosis. This  research was performed within the project POIG.01.01.02-00-048/09. Importantly, in my opinion for early diagnosis of AD, the determination of presence of only one marker is not enough. As the whole pathogenic process is extremely complex, we need to propose several molecules that will be examined. Indeed nowadays, it is β-amyloid that is most popularly tested as an early marker of the disease. The most important thing is to determine the ratio of its forms – monomeric and oligomeric. From the perspective of diagnosis, the process of oligomerization is of crucial importance as it can serve as an “alarm signal”. So now we are trying to investigate the relation between these two forms and propose how this can be used for diagnosis.

The basis of our method is the usage of ‘sensors” dedicated for particular molecules. Apart from β-amyloid, we also proposed other protein, S100B that is also associated with the development of AD. In general, as our strategy, we would like to provide tools enabling detection of several molecular markers and determination of their concentration as well as relation between them. Altogether, information derived from such results would give answer whether the disease is truly developing or not.

All the time, we are following the newest scientific reports and we are trying to find partners that would provide us markers for testing. Despite the fact that the AD field is developing really quickly, still the exact mechanism of the disease progression somehow remains mysterious. The number of researchers trying to resolve all the puzzles associated with AD is constantly growing, and we are trying to keep in touch with them in order to propose successful systems for an early diagnosis.

You mentioned that your detection method is based on „sensors”. Could you explain what they are?

Professor Hanna Radecka: In fact we are working with electrochemical sensors. We have chosen them because of several reasons. This technology requires very little sample volumes – we are operating in microliter volumes. Therefore, the amount of material needed for the analysis and in the same time the invasiveness of the whole method is really small. In addition, the costs of such “sensors” are not high. The target solution would be a device similar to that currently used for determination of blood glucose level, so portable potentiostat with disposable electrodes. To perform a test, a drop of blood or some of its fraction would be enough to obtain results. During our studies we observed that the “sensors” provide reliable results even in the presence of plasma. This is what we have achieved recently.

In the literature, a protein Tau is also usually mentioned considering AD. Can this protein be also useful for an early diagnosis of the disease?

Professor Hanna Radecka: Yes, of course. We are planning to elaborate a “sensor” for this marker. In our laboratory we have developed really good procedure of construction of “immunosensors” and “genosensors”. Concerning protein Tau, we would like to propose “immunosensor” for its detection. Here, as I mentioned earlier, the relation between the monomeric and oligomeric form is also critical. The aggregates of the protein are said to be most toxic for cells. Some of the oligomeric forms can be reversed to the monomeric form. Basing on this, we are searching for compounds that can prevent the process of oligomerization by using the  electrochemical “sensors”. In such a way we can monitor the process of aggregation in the presence of the particular groups of compounds. For example, in our laboratory we have examined various alkaloids that are found in spices. According to the obtained results, it is possible to provide preventive diet that would decrease the risk of AD onset.

Recently, there are a lot of reports talking about vaccines against AD. Do you think that such approach can be successful in the prophylaxis of this disease?

Professor Hanna Radecka: Yes, in my opinion these findings are very promising. Thanks to them, now we know that our organism is capable of defending against AD. Basing on this, the approach with application of vaccines was proposed in order to strengthen the reaction of the human organism. Such vaccines are already used in USA. The crucial thing is to apply them in a proper time, which is in an intermediate state of AD development. In this context, an early diagnosis would enable choosing the appropriate time of vaccination. We are inspired by all these finding to construct “sensors” detecting antibodies against monomeric and oligomeric forms of  β-amyloid. I really hope that it would be an efficient tool for the proper diagnosis. These are early beginnings of the studies on such “immunosensors”, however, we already have experience in that area and I think that we can provide the “sensors” in the nearest future.

If the test for early detection of AD works, patients will receive results saying that the disease is starting to develop. What are the possible ways of preventing or treating the AD progression?

Professor Hanna Radecka: It is still difficult question. I think that this enormous effort made by researchers all over the world will eventually provide some successful ways of AD treatment and we are trying to stay up-to-date with all the newest developments simultaneously presenting diagnosis methods. In my opinion studies on treatment and early diagnosis will meet together in some point and will complement each other. For now, the available ways of prophylaxis and treatment are quiet narrow encompassing mainly changes in lifestyle. For instance, as I mentioned earlier we can enrich our diet in components that can potentially hamper the process of AD development. It is also recommended to “train your brain”. One of the most popular exercises to keep your brain active is to learn foreign language in order to maintain a good condition of neuronal network. However, I am aware that such recommendations are not satisfying and effective enough. Nevertheless, I am sure that the great research potential will eventually result in earlier diagnosis and better therapies for AD.

by Maciej Smolarz

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Guest
2015-09-29 11:26:01 +0200
I am just another person that as i would say, the ' GREAT SPELL CASTER OTIS DARKO ' has helped word can not describe all he did for me i would rather you all that would read this were all in my life when all what he did for me materialized because only then will you really believe what i am talking about. I was not born with a royal blood and i do not even come from a decent family by decent i mean a family with wealth and all that and it was more than normal that my relationship with a royal blood will cause a lot of feud in both my home and that of my fiance. Yes he from a royal family but i will not mentions anything else in other to avoid scandal. We meant on a faithful Sunday in church and just like that he swept me off my feet i knew he was the prince but that was not while i fell for him it was more like i fell for him because he seem calm and always at else with everything it was like he did not even see himself as a prince. May be you may think its because it was not next in line but when i got to really know him, i saw that he was just an unembellished person that wanted to live his life in peace. It all start with us being friend and then on every night of Christmas 2004 that he spent at my house he kissed me and believe me in my mind i was Cinderella. It was like my heart was going to burst in flame i never knew i had all those feelings locked away in my heart but he broke the chain that was holding it back with just one kiss. Even when he wanted to be with me he family was again it and it was always like i was the root of what ever problem he was going through with his family. I thought to myself, i can not be the one to take the peace he had i felt he was better and at else without me. We where always of and on and all the while he waited for me with the biggest of hope that i will come back. I knew he was madly in love with me as i was in love with him but being with him was a problem as much as not being with him. His family won't let him be with with me. They opposed our relationship for nine years we where in love but unhappy our relationship was going no where with all the feud. I could no longer be the one an entire family hate and the same time be in love with a guy that his family did not want me. I some how some way got to know about OTIS DARKO a spell cast a great one at that. All i want was to be with my now fiance forever and to be loved and cared for by his family just as it suppose to be. I asked him to help me make my heart desires materialize because i saw a lot of testimonies on the internet about him and the number of people he helped everybody and every blog and forum had his name and his work. I needed to be one of the people he helped. After a lot of conversation he agreed to help me and asked that i get some materials he listed for me. I could get them but i would have made too many people ask question and it needed to be done in a very low profile so i wired the total cost to him so that he can get the materials i needed for the spell and there by avoid exposing myself over what should have be done in secret until the spell process was completed. Yes OTIS DARKO helped me with he spell and he send me a package containing what i can not disclose all i can say is that it was the result of the spell he helped me cast and he gave instruction on how i would use it to make the spell effective. I did all he asked of me and just like he promise me at the beginning, the spell made my fiance entire family love respect and treat me like their own child. To me i would say that OTIS DARKO was my fairy godmother because maybe right now my life would have be miserable basically because i would have had to let go of the love of my life because no one or more like his family would not approve our union. Contact OTIS DARKO because you will only be helping yourself and yourself only use this email as contact otisdarko60@yahoo.com
Guest
2015-09-25 04:25:56 +0200
I am very grateful to Dr. Bully The Mighty spell caster from India for helping me in my problem, I was almost losing hope in life when my lover of 4 years ended our relationship without any reason and I consulted an old friend of mine and explain my situation to her and she said she was in same situation 2weeks back but a wonderful man who was sent to help people helped her out of the problem and I thought it was all a joke and I ask her if it was real and she said if it wasn't real so how did her lover come back to her, then I decided to give Dr. Bully a try because I had nothing to loose in trying and I contacted him on his email address which is bullytemple@yahoo.com and he replied to my email very well.. and to cut the long story short Dr. Bully brought back my Lover in 48 hours.. I'm very happy he brought my man back to me and my lover promise that he will never and ever leave me again. Thanks to Dr. Bully of bullytemple@yahoo.com
Guest
2015-09-25 04:25:41 +0200
I am very grateful to Dr. Bully The Mighty spell caster from India for helping me in my problem, I was almost losing hope in life when my lover of 4 years ended our relationship without any reason and I consulted an old friend of mine and explain my situation to her and she said she was in same situation 2weeks back but a wonderful man who was sent to help people helped her out of the problem and I thought it was all a joke and I ask her if it was real and she said if it wasn't real so how did her lover come back to her, then I decided to give Dr. Bully a try because I had nothing to loose in trying and I contacted him on his email address which is bullytemple@yahoo.com and he replied to my email very well.. and to cut the long story short Dr. Bully brought back my Lover in 48 hours.. I'm very happy he brought my man back to me and my lover promise that he will never and ever leave me again. Thanks to Dr. Bully of bullytemple@yahoo.com
Guest
2015-09-15 03:32:16 +0200
Am very happy to tell every one to hear my testimony will say. am joy grecia from united state,am a nurse,this story of my love life.I have been married for 4years and on the fifth year of my marriage, another woman had to take my lover away from me and my husband left me and the kids and we have suffered for 2years until i met a post where this man Dr. ataso have helped someone and i decided to give him a try to help me bring my lover back home and believe me i just send my picture to him and that of my husband and after 48hours as he have told me, i saw a car drove into the house and behold it was my husband and he have come to me and the kids and that is why i am happy to make every one of you in similar to met with this man and have your lover back to your self.You can contact him with this email address {dr.atasohomeofsolution@gmail.com} or you can still call or Whatsapp him on his mobile +2348077922457 Thank you Dr ataso.
Guest
2015-08-30 09:30:15 +0200
i want to use this opportunity to thank Dr Ofemo who he really made my life a pleasurable one today,i read some testimony about Dr Ofemo on how he has helped people in bringing back there ex within 24hours, i was just thinking if that was real,he help Janet to bring is ex lover back,And decided to call Janet who he help made a testimony and also dropped her number,So i called her and ask her about Dr Ofemo she said Dr Ofemo is a trustworthy man and he his ready to bring back my lover for me,i was just so happy and a little bit relief that my ex boyfriend will be back to me soon,Then i decided in contacting Dr Ofemo which i did,And before i could share him my problem he has already told me what i came for,And he said everything will be okay within 24hours that my ex boyfriend will be back to my arms,So he said he would be casting the spell and that within 24hours my ex boyfriend would call me,So i hoped so truly before the 24hours i got a call from my ex boyfriend who has left me for the past 5 years saying he is sorry and he wants me back,i was happy and i said i also want him back,we are happy with ourselves. Thanks to Dr Ofemo for saving my relationship and for also saving others too.continue your good work, If you are interested to contact him and testify this blessings like me,contacted him drofemospelltemple@gmail.com or cell number +2348163387496 contact Dr Ofemo is the answer of all problem via drofemospelltemple@yahoo.com or his web/site: http://drofemospelltemple.wix.com/dr-ofemo
Guest
2015-08-26 17:52:46 +0200
my heart is overwhelmed with Joy.that is why i am advising any one out there to contact Dr Bully, the mighty Psychic that can help you reunite you and your ex lover in just 48 hours...i don't have much to say but i must thank Dr Bully for helping me reunite my marriage with my Ex Husband in 48 hours when i taught nothing could be done any more...he is the best, genuine and Great Psychic that can help you solve any relationship problem, getting pregnant and even help you Cure Any Disease with his Natural Power....his spell work within 48 hours and it has no side effect...you can contact Dr Bully through his email at bullytemple@yahoo.com..you can also add him on Facebook as "Kareem Abhida" or message him through his page on Facebook as "Dr Bully Temple"""..his website is bullyhelptemple.webs.com.once again his email address is bullytemple@yahoo.com contact him now and get your problem solved for ever...
Guest
2015-08-26 17:52:37 +0200
my heart is overwhelmed with Joy.that is why i am advising any one out there to contact Dr Bully, the mighty Psychic that can help you reunite you and your ex lover in just 48 hours...i don't have much to say but i must thank Dr Bully for helping me reunite my marriage with my Ex Husband in 48 hours when i taught nothing could be done any more...he is the best, genuine and Great Psychic that can help you solve any relationship problem, getting pregnant and even help you Cure Any Disease with his Natural Power....his spell work within 48 hours and it has no side effect...you can contact Dr Bully through his email at bullytemple@yahoo.com..you can also add him on Facebook as "Kareem Abhida" or message him through his page on Facebook as "Dr Bully Temple"""..his website is bullyhelptemple.webs.com.once again his email address is bullytemple@yahoo.com contact him now and get your problem solved for ever...
Guest
2015-08-26 17:52:29 +0200
my heart is overwhelmed with Joy.that is why i am advising any one out there to contact Dr Bully, the mighty Psychic that can help you reunite you and your ex lover in just 48 hours...i don't have much to say but i must thank Dr Bully for helping me reunite my marriage with my Ex Husband in 48 hours when i taught nothing could be done any more...he is the best, genuine and Great Psychic that can help you solve any relationship problem, getting pregnant and even help you Cure Any Disease with his Natural Power....his spell work within 48 hours and it has no side effect...you can contact Dr Bully through his email at bullytemple@yahoo.com..you can also add him on Facebook as "Kareem Abhida" or message him through his page on Facebook as "Dr Bully Temple"""..his website is bullyhelptemple.webs.com.once again his email address is bullytemple@yahoo.com contact him now and get your problem solved for ever...
Guest
2015-08-26 17:52:20 +0200
my heart is overwhelmed with Joy.that is why i am advising any one out there to contact Dr Bully, the mighty Psychic that can help you reunite you and your ex lover in just 48 hours...i don't have much to say but i must thank Dr Bully for helping me reunite my marriage with my Ex Husband in 48 hours when i taught nothing could be done any more...he is the best, genuine and Great Psychic that can help you solve any relationship problem, getting pregnant and even help you Cure Any Disease with his Natural Power....his spell work within 48 hours and it has no side effect...you can contact Dr Bully through his email at bullytemple@yahoo.com..you can also add him on Facebook as "Kareem Abhida" or message him through his page on Facebook as "Dr Bully Temple"""..his website is bullyhelptemple.webs.com.once again his email address is bullytemple@yahoo.com contact him now and get your problem solved for ever...
Guest
2015-08-26 17:52:09 +0200
my heart is overwhelmed with Joy.that is why i am advising any one out there to contact Dr Bully, the mighty Psychic that can help you reunite you and your ex lover in just 48 hours...i don't have much to say but i must thank Dr Bully for helping me reunite my marriage with my Ex Husband in 48 hours when i taught nothing could be done any more...he is the best, genuine and Great Psychic that can help you solve any relationship problem, getting pregnant and even help you Cure Any Disease with his Natural Power....his spell work within 48 hours and it has no side effect...you can contact Dr Bully through his email at bullytemple@yahoo.com..you can also add him on Facebook as "Kareem Abhida" or message him through his page on Facebook as "Dr Bully Temple"""..his website is bullyhelptemple.webs.com.once again his email address is bullytemple@yahoo.com contact him now and get your problem solved for ever...
Guest
2015-08-23 15:15:17 +0200
Hello viewers i am so happy to share this testimony to you all in different part of the the world. my name is Duane. on how i won lottery with help of Dr Friday. on a faithful day as i was walking down my street, when i meet my old friend Randall rush he was so happy to see me and he pick me up to a fast food joint in the city of new York USA, he was my old time friend and class mate, but now he look different absolution look good riding a big car and wearing all sort of customized Golding wristwatch and necklace. i was jealous but since he is my very good friend i have to ask him some few question on how come about his wealth and he say to me as a good friend and old friend of him, he told me the secret of his wealth behold i was so surprise that a voodoo spell caster can cast a lottery spell and will send you the hot numbers to win millions of dollars, i try my best to make sure i get good money and take care of my family, so believe me i contacted this Africa voodoo spell caster name Dr friday, i contacted him quickly through his mail address at drfridayspelltemple@yahoo.com and he cast the spell and send me the hot numbers to win the lottery big, to cut the long story short i am a billionaire today, Dr friday is truly a gifted voodoo spell caster and you can contact him today for a lottery spell. because this is the only way to win the lottery and the best way. if you want to win lottery contact him on his email now on line: drfridayspelltemple@gmail.com or drfridayspelltmeple@outlook.com or tell:+2349034015376
Guest
2015-08-23 15:15:06 +0200
Hello viewers i am so happy to share this testimony to you all in different part of the the world. my name is Duane. on how i won lottery with help of Dr Friday. on a faithful day as i was walking down my street, when i meet my old friend Randall rush he was so happy to see me and he pick me up to a fast food joint in the city of new York USA, he was my old time friend and class mate, but now he look different absolution look good riding a big car and wearing all sort of customized Golding wristwatch and necklace. i was jealous but since he is my very good friend i have to ask him some few question on how come about his wealth and he say to me as a good friend and old friend of him, he told me the secret of his wealth behold i was so surprise that a voodoo spell caster can cast a lottery spell and will send you the hot numbers to win millions of dollars, i try my best to make sure i get good money and take care of my family, so believe me i contacted this Africa voodoo spell caster name Dr friday, i contacted him quickly through his mail address at drfridayspelltemple@yahoo.com and he cast the spell and send me the hot numbers to win the lottery big, to cut the long story short i am a billionaire today, Dr friday is truly a gifted voodoo spell caster and you can contact him today for a lottery spell. because this is the only way to win the lottery and the best way. if you want to win lottery contact him on his email now on line: drfridayspelltemple@gmail.com or drfridayspelltmeple@outlook.com or tell:+2349034015376
Guest
2015-08-23 12:12:03 +0200
WONDERS NEVER END!!WONDERS NEVER END My Name is Allan Peek. I Am from Australia I will love to share my testimony to all the people in the forum because i never thought i will have my girlfriend back and she means so much to me..The girl i want to get marry to left me 4 weeks to our wedding for another man..,When i called her she never picked my calls,She deleted me on her Facebook and she changed her Facebook status from engage to Single…when i went to her place of work she told her boss she never want to see me..I lost my job as a result of this cos i cant get myself anymore,my life was upside down and everything did not go smooth with my life…I tried all i could do to have her back to all did not work out until i met a Man when i Travel to Germany to execute some business have been developing some years back..I told him my problem and all have passed through in getting her back and how i lost my job…he told me he gonna help me…i don’t believe that in the first place.but he swore he will help me out and he told me the reason why my girlfriend left me and also told me some hidden secrets.i was amazed when i heard that from him..he said he will cast a spell for me and i will see the results in the next couple of days..then i travel back to Germany the following day and i called him when i got home and he said he’s busy casting those spells and he has bought all the materials needed for the spells,he said am gonna see positive results in the next 2 days that is Thursday.My girlfriend called me at exactly 12:42pm on Thursday and apologies for all she had done ..she said,she never knew what she’s doing and her sudden behavior was not intentional and she promised not to do that again.it was like am dreaming when i heard that from her and when we ended the call,i called the man and told him my my girl friend called and he said i haven’t seen anything yet… he said i will also get my job back in 3 days time..and when its Sunday,they called me at my place of work that i should resume work on Monday and they gonna compensate me for the time limit i have spent at home without working..My life is back into shape,i have my girlfriend back and we are happily married now with a baby boy and i have my job back too.This man is really powerful..if we have up to 20 people like him in the world,the world would have been a better place..he has also helped many of my friends to solve many problems and they are all happy now..Am posting this to the forum for anybody that is interested in meeting the man for help.you can reach him on Email;Erigospellcaster@gmail.com
Guest
2015-08-18 09:45:34 +0200
For the past 3 months now I have been looking for good and geniue spell to bring back my ex lover back and also help me to win lottery. and it has been a hard time for me finding the right place until I get in contact with Dr Alika who gave me powerful prayer and assured me that it will take two days before the spell will be effective, truly 2 days later, my Ex Lover rang me, i answered the call, he started apologizing, we are together Now. Thank you for making her to know that we are meet to be together and also making my dreams come true. And he did a spell that make me to win lottery. Please if any body needs LOVE SPELL,LOTTERY,PREGNANCY SPELL, DIVORCE SPELL, GET YOUR PARTNER. BACK AND MANY MORE YOU MAKE NEED. Email him now for your own help. lordalikaspelltemple@yahoo.co.uk or http://lordalikaspelltemp8.wix.com/http call him +2348072718865 All thanks goes to DR.Alika for the excessive work that he has done for me.
Guest
2015-08-07 07:34:28 +0200
i am so very happy to testify about this great spell caster Dr Okaya that help me cured my HIV disease. I have been suffering from this HIV disease for over 2 years and i have found know cure, on till this blessed day i was browsing through the internet and i saw a woman testifying about this great powerful spell caster Dr OKAYA who helped her cured her HIV disease with a herbal cure. And also i contacted him Dr ,and i explain all my problem to him and he told me that i should not worry that he is going to help me prepare a herbal medicine for me and after that, he told me that he will send me a oil that i will use in curing my HIV disease and on the oil there are some rules that i will follow in using the oil, and i stood by it, and after using the oil i felt changing in me and i went for medical check up and the doctor told me that i am cured from the HIV disease and i was so very happy now that i am free from HIV disease and also the woman mentioned that Dr OKAYA can also cure this deadly diseases called Cancer, Diabetes, Syphilis, Herpes, i am now saying that anyone who is suffering from any of this deadly disease should contact this powerful great spell caster at his email: OKAYASPELLHELP@OUTLOOK.COM or call his Moblie number:+2348078291904 he is the right one that can help you from any of the diseases contact him:web site: http://okayaspelllhelp.webs.com
Guest
2015-07-29 07:32:05 +0200
I am writing this because I want to testify how great you are Priest Ola for what you have done for me. Everyone needs to know! I am a very satisfied and happy customer. I can’t tell how long it’s been since I’ve bought spells online, but none of them had the same results than yours. Fuller definitely moved in with me 1 week ago. It was just a few days after you cast your spell. I know I should have messaged you before, but I was a bit scared that he could find out about the spell you did so I removed all your emails. Anyway, I can tell all your future customers who are reading this that they are doing the right choice to go with you for a spell. It was always great communicate with you and all the services you offer are just excellent. Thank you for everything you did for me!” Fuller USA. CONTACT Priest Ola on successspells@outlook.com or successspells@yahoo.com. Or call his telephone number on +1 440 607 5300
Guest
2015-07-21 07:19:04 +0200
Since Dr.Adodo of Adodospelltemple@gmail.com cast a love spell for me, things are going great in my relationship life. my lover who was cheating on me is now a loyal and committed man. I`m glad he came back to me after the break up with him because I love him, but without Dr.Adodo help, all of this could not have happen or even be possible. It is the first time I am using the service of a spell caster and even if I was a bit skeptical at first, I highly recommend his service to people like me who needs an extra help. All they should do is to contact Dr.Adodo on +2348156784928 or better still reach him on his email on Adodospelltemple@gmail.com or on web http://adodospelltemple.wix.com/adodospelltemple
Guest
2015-07-18 22:29:30 +0200
My name is Kimberly and i base in U.S.A…“My life is back!!! After 1 years of Broken marriage, my husband left me with two kids . I felt like my life was about to end i almost committed suicide, i was emotionally down for a very long time. Thanks to a spell caster called Dr.Zack Balo which i met online. On one faithful day, as i was browsing through the internet,I came across allot of testimonies about this particular spell caster. Some people testified that he brought their Ex lover back, some testified that he restores womb,cure HIV,and other sickness, some testified that he can cast a spell to stop divorce and so on. i also come across one particular testimony,it was about a woman called Melissa,she testified about how he brought back her Ex lover in less than 3 days, and at the end of her testimony she gave Dr.Zack Balo ’s e-mail address. After reading all these,I decided to give it a try. I contacted him via email and explained my problem to him. In just 3 days, my husband came back to me. We solved our issues, and we are even happier than before. Dr.Zack Balo is really a gifted man and i will not stop publishing him because he is a wonderful man… If you have a problem and you are looking for a real and genuine spell caster to solve all your problems for you. Try Dr.Zack Balo anytime, he might be the answer to your problems. Here’s his contact: wiseindividualspell@gmail.com. or call his phone number +2348078927387 .
Guest
2015-07-17 21:08:50 +0200
I so much thank God that I found Dr.Adodo’s contact email through testimonies i found on some blogs. He helped me and assured me that my boyfriend will come back to me within the period of 48 hours and today makes it two weeks (14 days) that my lover came to me all through the help of Dr.Adodo. Please people don't waste your time in seeking for help in the wrong places just contact Dr.Adodo at Adodospelltemple@gmail.com or via mobile on +2348156784928 and get your lover back within 48 hours. Thanks Dr.Adodo, or contact him on his website http://adodospelltemple.wix.com/adodospelltemple
Guest
2015-07-17 09:32:47 +0200
My name is Gloria from Sweden, My boyfriend left me a month ago and he was leaving with another woman who is 7 years older than him,i feel like my life is completely over so one day as i was browsing searching on the internet for help I read over the internet how a spell caster have help several people to get there love back. I have been depress for the past one month and what i need is to get him back and live with him happily. so i decided to give it a try so i contacted the spell caster called Dr.Adodo and explain my problems to him and he cast a love spell which i use to get my boyfriend back and now my life is complete and both of us are very happy with the relationship. and i am really grateful to this man for what he has done for me and my family, Thank you very much and i will continue to say my testimony over and over again. for those you need help or having same problem as me should contact him on his email address Adodospelltemple@gmail.com or call his mobile number +2348156784928 or through web http://adodospelltemple.wix.com/adodospelltemple
Guest
2015-07-17 08:13:22 +0200
i am Helen and i want to thank Dr.otonu for bringing back my ex boyfriend, we broke up for more than 8 month and he told me that he will never want to see me in his life again. i love him so much to the extend that i could not think of dating any man again, i was confused and depress due to the love i had for him,i did everything i could do to have him come back to me but all went in vain. so i decided to contact a spell caster, i did not believe in spell casting i just want to try it may be it would work out for me. i contacted Dr.otonu for help,and he told me that he have to cast a love spell on him, i told him to do it. after 5 days my boyfriend called me and started to apologize for leaving me and also he told me that he still love me. i was very happy and i thank Dr otonu for helping me get back my ex back to my hands. his spell is the greatest of all over the world, it was the love spell he cast on my ex that make him come back to me. all you ladies who want back their ex back i want you to contact Dr.otonu for the return of your ex boyfriend and also your ex girlfriend he can also cast any kind of spell you want him to cast for you. his contact email is( otonuspelltemple@outlook.com) or (otonuspelltemple@gmail.com) or call him on telephone (+2348169616855) just try him and there will be a solution to your case.
Guest
2015-07-16 23:12:31 +0200
Greeting to everyone that is reading my comment, All Thanks goes to dr.Laco, i was married to my husband, and we were living fine and happy. it come to an extend that my husband that use to love and care for me, those not have my time again, until i fined at that he was having an affair with another woman, i try to stop him,all my effort was in-vain suddenly he divorce me and went for the woman. he live me with two of our kids, i cry all day, i was in pains, sorrow and looking for help. i read a comment on how he helped a lady to brought her husband back how after 2yrs , i saw how dr.Laco help people with his love and reuniting spell. so i decided to contact him and explain my problem to him, he did a love spell that make my husband to come back to me and our kids and never think of the woman. this man is God sent to restore heart break and reunite relationship. may the lord be your strength and continue to use you to save people relationship and any problem they encounter contact him for help on lacopowerfulspellcaster@yahoo.com ,i promise you that he will put a smile on your face and make you feel happy. or +2348072718865 if you are searching for any help to problems like these: (1)If you want your ex back. (2)if you always have bad dreams. (3)You want to be promoted in your office. (4)You want women/ men to run after you. (5)If you want a child. (6)You want to be rich. (7)You want to speak to your late ones (8)If you need financial assistance. (9)Herbal care
Guest
2015-07-14 00:32:07 +0200
My name is Isabella, from united state, i want to share my wonderful testimony on how i got back the love of my life, i want to tell everyone that there is a real spell caster online and is powerful and genuine, His name is Dr.Adodo, He helped me to reunite relationship with my boyfriend who dumped me after having sex with me, When i contacted dr Adodo, he cast a love spell for me and my lover who said he doesn't have anything to do with me again called me and started begging me.To anyone who is reading this article and needs any help, Dr.Adodo can also offer any types of help like Reuniting of marriage and relationship, Curing of Diseases, Court Cases, Pregnancy Spell, Spiritual protection and lot's more.thanks once again wonderful man for helping me to bring my ex lover back to me, thank you again and again Dr.Adodo, You can contact him Via this email: [Adodospelltemple@gmail.com] or call him on his mobile number +2348156784928! you can also contact him on his website http://adodospelltemple.wix.com/adodospelltemple
Guest
2015-07-12 07:04:17 +0200
My name is Ethan Mia. I want to give thanks to DR. Adodo, for bringing back my husband,No one could have ever made me believe that the letter I’m about to write would actually one day be written. I was the world’s biggest skeptic. I never believed in magic spells or anything like this, but I was told by a reliable source (a very close co-worker) that DR Adodo is a very dedicated, gifted, and a medicine man, It was one of the best things I have ever done. My love life was in shambles; I had been through two divorces and was on the brink of third one. I just couldn't face another divorce, and I wanted to try harder to make our relationship work, but my husband didn’t seem to care. and he broke up with me again, I was confuse and do not know what to do again, rather than to get in contact with DR Adodo. He did a love spell that make my husband come back to me. we are now very much happy with our self. DR. Adodo make him to realize how much we love and need each other.This man is for REAL and for good.he can also help you to fix your broken relationship. I had my husband back! It was like a miracle! He suddenly wanted to go to marriage counseling, and we’re doing very very well in our love life.contact DR.Adodo today so that he will help you restore you lost Joy and Lover back to you with his email address: Adodospelltemple@gmail.com or through phone number:+2348156784928! you can also visit his website http://adodospelltemple.wix.com/adodospelltemple
Guest
2015-07-09 09:39:36 +0200
I decided to create time for myself to enable me thank this great man that made the restoration of my relationship a success, Let me start by introducing myself, I am Gina from Finland and i came to this site with one purpose on my mind which is to thank the great man that restored my relationship called Dr.Adodo within the space of 48 hours. I must say that since the help of this great man my relationship has been on a safer edge.. This is the reason why i want other people out there to have a taste of Dr.Adodo's power. The best way to contact Dr.Adodo are +2348156784928 or email him on Adodospelltemple@gmail.com or on his website http://adodospelltemple.wix.com/adodospelltemple
Guest
2015-07-04 07:03:29 +0200
Hello friends, there is a powerful and genuine Love spell to restore broken relationships and marriage, My name is Natasha Bella. i live in UK, and I'm happily married to a lovely and caring husband ,with three kids. A very big problem occurred in my family seven months ago,between me and my husband .so terrible that he took the case to court for a divorce.he said that he never wanted to stay with me again,and that he didn't love me anymore.So he packed out of the house and made me and my children passed through severe pain. I tried all my possible means to get him back,after much begging,but all to no avail.and he confirmed it that he has made his decision,and he never wanted to see me again. So on one evening,as i was coming back from work,i met an old friend of mine who asked of my husband .So i explained every thing to him,so he told me that the only way i can get my husband back,is to visit a spell caster,because it has really worked for him too.So i never believed in spell,but i had no other choice,than to follow his advice. Then he gave me the email address of the spell caster whom he visited.{bravespellcaster@gmail.com}. So the next morning,i sent a mail to the address he gave to me,and the spell caster assured me that i will get my husband back the next day.What an amazing statement!! I never believed,so he spoke with me,and told me everything that i need to do. Then the next morning, So surprisingly, my husband who didn't call me for the past seven {8}months,gave me a call to inform me that he was coming back.So Amazing!! So that was how he came back that same day,with lots of love and joy,and he apologized for his mistake,and for the pain he caused me and my children. Then from that day,our relationship was now stronger than how it were before,by the help of a spell caster. So, i will advice you out there to kindly visit the same website { http://lovespelldrbrave.weebly.com/} and you can also reach Dr Brave through his mobile number +2348072370762,if you are in any condition like this or you have any problem contact Dr Brave ,{ bravespellcaster@gmail.com }, Thanks for reading.
Guest
2015-07-03 22:41:58 +0200
Hello to every one i want to testify of what a spell caster has done for me,i was in a relationship for two years happy with my partner few months to our wedding she left me and went after another man she never came back home again, i cry all day looking for help i have contacted so many spell caster but no result one day i was on the internet when i came across a testimony on how Doctor Jatto helped some one to get back his lover so i gave a try and it work out for me am so happy to have my lover back to my self again thanks once again Doctor Jatto for helping me to bring back my lover if you are passing through the same problem or any kind of situation and you think all hope is lost my dear is not. contact Doctor Jatto on his email : (drjattosplltemple@gmail.com). OR you can call the great man on +2349035512062. my name is Juan Rohit
Guest
2015-06-29 13:10:05 +0200
Thanks to all those out there who in one way or the other has been able to let the world know about this powerful spell caster called DR.Adodo. I was just searching the internet for some stuff then i came across an article about how Dr.Adodo was able to save and restore broken relationship. Before i came across the article i have been facing hard times in my marriage since my husband packed his things out of the house, So i contacted Dr.Adodo through these details via email: Adodospelltemple@gmail.com and via mobile number +2348156784928 that i copied out of the article. I must say that me contacting Dr.Adodo really worth it because through the help of Dr.Adodo my husband came back home and was pleading for our reunion. If you are interested in reaching Dr.Adodo just contact him through those details above or through his website http://adodospelltemple.wix.com/adodospelltemple
Guest
2015-06-24 03:18:36 +0200
My name is murphy chloe, i live and work in Florida, US. My life is back!!! After 2 years of Broken marriage, my husband left me with two kids, I felt like ending it all, i almost committed suicide because he left us with nothing, i was emotionally down all this while. Thanks to a spell caster called lord masuka the spell caster which i met online. On one faithful day, as I was browsing through the internet, I came across several of testimonies about this particular spell caster. Some people testified that he brought their Ex lover back, some testified that he restores womb,cure cancer,and other sickness, some testified that he can cast a spell to stop divorce and also spell to get a good paid job so on. He is amazing, i also come across one particular testimony, it was about a woman called bernice, she testified about how he brought back her Ex lover in less than 2 days, and at the end of her testimony she dropped his email. After reading all these, I decided to give it a try. I contacted him via email and explained my problem to him. In just 48hours, my husband came back to me. We resolved our issues, and we are even happier than ever lord masuka you are a gifted man and i will not stop publishing him because he is a wonderful man. If you have a problem and you are looking for a real and genuine spell caster, Try him anytime, he is the answer to your problems. you can contact him on lordmasukaspelltemple@hotmail.com or via his website http://lordmasukalovespelltemple.webs.com or call him +2347053105287. He is the best caster that can help you with your problems.
Guest
2015-06-24 03:18:21 +0200
My name is murphy chloe, i live and work in Florida, US. My life is back!!! After 2 years of Broken marriage, my husband left me with two kids, I felt like ending it all, i almost committed suicide because he left us with nothing, i was emotionally down all this while. Thanks to a spell caster called lord masuka the spell caster which i met online. On one faithful day, as I was browsing through the internet, I came across several of testimonies about this particular spell caster. Some people testified that he brought their Ex lover back, some testified that he restores womb,cure cancer,and other sickness, some testified that he can cast a spell to stop divorce and also spell to get a good paid job so on. He is amazing, i also come across one particular testimony, it was about a woman called bernice, she testified about how he brought back her Ex lover in less than 2 days, and at the end of her testimony she dropped his email. After reading all these, I decided to give it a try. I contacted him via email and explained my problem to him. In just 48hours, my husband came back to me. We resolved our issues, and we are even happier than ever lord masuka you are a gifted man and i will not stop publishing him because he is a wonderful man. If you have a problem and you are looking for a real and genuine spell caster, Try him anytime, he is the answer to your problems. you can contact him on lordmasukaspelltemple@hotmail.com or via his website http://lordmasukalovespelltemple.webs.com or call him +2347053105287. He is the best caster that can help you with your problems.
Guest
2015-06-18 01:18:00 +0200
I HAVE FOLLOWED MOST OF THESE CRAPS AND GOT SCAMMED. I EVEN SEE SOME OF THEM REWRITING WHAT I WROTE ON THE NET. MOST OF THESE PEOPLE TELLING YOU FAKE TESTIMONIES AND FOR YOU TO BEWARE OF THIS AND BEWARE OF THAT ARE BUNCH OF CRAPS AND SCAMS. THE ONLY MAN YOU CAN ACTUALLY TRUST ON SPELL IS DR MALAIKA HIS EMAIL IS odogwumalaika@gmail.com, I WOULD HAVE BROUGHT HIM RIGHT HERE IN THE STATES IF POSSIBLE. HE DID A WHOLE LOT OF THINGS WHICH I WON’T REALLY MENTION HERE BUT THE MOST OF WHAT HE DID FOR ME WAS HELP ME OUT OF CANCER AND ALSO HELPED ME GOT BACK MY JOB AND MY MARRIAGE. HE IS GREAT AND I MET HIM OVER THE INTERNET JUST AS EVERY OTHER PERSON HAS TESTIFIED BUT HE IS ACTUALLY REAL AND A VERY GOOD SPELL CASTER. I’M STILL VERY MUCH INDEBTED TO DR MALAIKA FOR SAVING MY LIFE AND MY MARRIAGE. I WANT TO LET YA ALL KNOW THAT YOU DON’T HAVE TO TRY ANYBODY ELSE EXCEPT THIS DUDE. HIS EMAIL CONTACT IS odogwumalaika@gmail.com THE MOBILE IS +2347065448120
Guest
2015-06-03 07:53:39 +0200
My name is Laurie Jonathan, I live in Canada want to share my wonderful testimony on how i got back my ex-lover of my life back, who i sworn to be with until when he left me to another woman for no reason and i try to make things work for both of us yet things where getting worse and i love him so much and there is nothing i could do to get my ex back until i met a testimony share by Maria from USA on the internet talking about a powerful spell caster who brought his ex lover back within 48 hours and i decide to give it a try and to my greatest surprise he also did it for me just as he did for Maria and i have a lot of people complaining of fake spell caster but this one i met was a real spell caster who help me to solve my problem i have no solution to,i introduce many of my best friends that have a similar problems,and their problem were solve with the great help of Dr.Iyaryi. they get back their ex within 48 hours. I am so happy that my ex is back to me again,and the most surprise,is that our love is very strong,every day is happiness and joy. and there is nothing like been with the man you love. I am so happy my love is back to me with the help of Dr.Iyaryi. if you have similar problem i will advice you to contact him ,he is there to help you and put a smile on your face ask he did to me and others.contact email(driayaryi2012@hotmail.com)
Mccart2
2015-05-17 14:02:56 +0200
Am here to testify what this great spell caster done for me. i never believe in spell casting, until when i was was tempted to try it. i and my husband have been having a lot of problem living together, he will always not make me happy because he have fallen in love with another lady outside our relationship, i tried my best to make sure that my husband leave this woman but the more i talk to him the more he makes me fell sad, so my marriage is now leading to divorce because he no longer gives me attention. so with all this pain and agony, i decided to contact this spell caster to see if things can work out between me and my husband again. this spell caster who was a woman told me that my husband is really under a great spell that he have been charm by some magic, so she told me that she was going to make all things normal back. she did the spell on my husband and after 5 days my husband changed completely he even apologize with the way he treated me that he was not him self, i really thank this woman her name is Dr Aluta she have bring back my husband back to me i want you all to contact her who are having any problem related to marriage issue and relationship problem she will solve it for you. her email is traditionalspellhospital@gmail.com she is a woman and she is great. wish you good time. He cast spells for different purposes like (1) If you want your ex back. (2) if you always have bad dream (3) You want to be promoted in your office. (4) You want women/men to run after you. (5) If you want a child. (6) You want to be rich. (7) You want to tie your husband/wife to be yours forever. (8) If you need financial assistance. (9) HIV/AIDS CURE (10) is the only answer to that your problem of winning the lottery Contact him today on: traditionalspellhospital@gmail.com
Haydenrhoades
2015-05-15 11:23:09 +0200
[url=http://www.sargaiar.com]sargaiar.com[/url]
Haydenrhoades
2015-05-15 11:22:29 +0200
Go here and take help <a href="http://www.sargaiar.com">sargaiar.com</a>
Guest
2015-05-13 13:01:57 +0200
An amazing testimony on how to get lost love back even if you made some mistakes, My name is Elizabeth Williams and i live in UK, and I'm happily married to a lovely and caring husband ,with two kids. A very big problem occurred in my family seven months ago,between me and my husband .so terrible that he took the case to court for a divorce.he said that he never wanted to stay with me again,and that he didn't love me anymore.So he packed out of the house and made me and my children passed through severe pain. I tried all my possible means to get him back,after much begging,but all to no avail.and he confirmed it that he has made his decision,and he never wanted to see me again. So on one evening,as i was coming back from work,i met an old friend of mine who asked of my husband .So i explained every thing to him,so he told me that the only way i can get my husband back,is to visit a spell caster,because it has really worked for him too.So i never believed in spell,but i had no other choice,than to follow his advice. Then he gave me the email address of the spell caster whom he visited.{bravespellcaster@gmail.com}. So the next morning,i sent a mail to the address he gave to me,and the spell caster assured me that i will get my husband back the next day.What an amazing statement!! I never believed,so he spoke with me,and told me everything that i need to do. Then the next morning, So surprisingly, my husband who didn't call me for the past seven {8}months,gave me a call to inform me that he was coming back.So Amazing!! So that was how he came back that same day,with lots of love and joy,and he apologized for his mistake,and for the pain he caused me and my children. Then from that day,our relationship was now stronger than how it were before,by the help of a spell caster. So, i will advice you out there to kindly visit the same website { http://enchantedscents.tripod.com/lovespell/} and you can also reach Dr Brave through his mobile number +2348072370762,if you are in any condition like this,or you have any problem related to "bringing your ex back. So thanks to the Dr Brave for bringing back my husband ,and brought great joy to my family once again. { bravespellcaster@gmail.com }, ╱◥◣ │∩ │◥███◣ ╱◥███◣ ╱◥◣ ◥████◣▓∩▓│ͪ 5; ║ │╱◥█◣║∩∩∩ ║◥█▓ ▓█◣Thanks..
Mccart2
2015-04-27 21:54:13 +0200
How to get lost love back even if you made some mistakes Am here to testify what this great spell caster done for me. i never believe in spell casting, until when i was was tempted to try it. i and my husband have been having a lot of problem living together, he will always not make me happy because he have fallen in love with another lady outside our relationship, i tried my best to make sure that my husband leave this woman but the more i talk to him the more he makes me fell sad, so my marriage is now leading to divorce because he no longer gives me attention. so with all this pain and agony, i decided to contact this spell caster to see if things can work out between me and my husband again. this spell caster who was a woman told me that my husband is really under a great spell that he have been charm by some magic, so she told me that she was going to make all things normal back. she did the spell on my husband and after 5 days my husband changed completely he even apologize with the way he treated me that he was not him self, i really thank this woman her name is Dr Aluta she have bring back my husband back to me i want you all to contact her who are having any problem related to marriage issue and relationship problem she will solve it for you. her email is traditionalspellhospital@gmail.com she is a woman and she is great. wish you good time. He cast spells for different purposes like (1) If you want your ex back. (2) if you always have bad dream (3) You want to be promoted in your office. (4) You want women/men to run after you. (5) If you want a child. (6) You want to be rich. (7) You want to tie your husband/wife to be yours forever. (8) If you need financial assistance. (9) HIV/AIDS CURE (10) is the only answer to that your problem of winning the lottery Contact him today on: traditionalspellhospital@gmail.com
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