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Unculturable microorganisms – the source of new antibiotics

Unculturable microorganisms – the source of new antibiotics

Publish date: 2016-01-18

The neuralgic period of antibiotic therapy history in which we are now prompts to intense search for new natural antibiotics, for example in environmental samples. The main areas of such search are sea water and soil. However, among plenty of microorganisms living in natural environment only 1% of strains are those which we can isolate and grow in laboratory. The capabilities of the rest 99% still remain uncovered.

Tags: natural antibiotics , VBNC , antibiotics , teixobactin

Water and soil full of living microorganisms are the major areas of the search for new species and metabolites of antimicrobial action which could be used against human pathogens or in biocontrol of plant infections. Around 80% of currently used antibiotics has been derived from soil microorganisms. For example, actinomycetes are the primary source of compounds such as streptomycin, actinomycin, erythromycin and vancomycin. In the time of growing resistance to known antibiotics it is a high time to come back to the source.

Microbiologic analysis of various soil types revealed, that samples from residential (urbanized) area differs from recreational soil as regards the quantity of species and produced metabolites. Flora in residential soil reveals higher metabolic activity due to constant exposition to substances and wastes from households or farms – such as feaces, food waste and pharmaceuticals (Woappi et al. 2013). In such environment the percentage of resistant strains is 10-20 times higher than in recreational or industrial soil. The horizontal transfer of resistance genes allows for co-existence of strains producing active compounds and resistant strains. This dynamics creates the suitable environment for evolutionary selection of metabolically active strains – due to maintain the balance between species, a selective pressure forces the synthesis of new active compounds and biocontrol of resistant ones. It is therefore the unlimited source of new potent antibiotics.

Unfortunately, standard screening methods for antimicrobial substances which are based mainly on inhibition zones determination, are limited by the need to have particular producing strain isolated. A percentage of species culturable at laboratory conditions is only 1%. The rest 99% is a population of viable but not culturable forms (VBNC). The group of researchers leaded by Prof. Kim Lewis from Northeastern University, Boston, MA (USA) Has find the way to get to this hidden part of iceberg. They employed the iChip (NovoBiotic Pharmaceuticals), a special cultivation device made of a rectangular 7 cm long plate with small wells coved by semi-permeable layer. Flora isolated from soil sample was diluted and loaded in a way that each well contained one microbial cell. Then such prepared iChip has been… buried back in a place of soil sampling, thus on a grass field in Maine, USA.

Ref. Ling et al. 2015

Microorganisms which were grown in such natural environment supplied with soil components, have been tested for the production of antibiotics against human pathogens. This is how teixobactin was discovered – a compound inhibiting bacterial cell wall synthesis by binding to conserved motifs of lipid II (a precursor of peptidoglycan) and lipid III (a precursor of wall teichoic acids). A structure of teixobactin and the predicted biosynthetic gene luster Has been published last year in Nature (Ling et al. 2015). This antibiotic, due to the lack of detectible resistance induction in Staphylococcus aureus and Mycobacterium tuberculosis, is currently a major candidate for clinical application.

The knowledge of active substances and their encoding genes allows for use of other detection methods in situ – such as real time PCR. As a very sensitive method, it allows the amplification of genes of interest in DNA isolated directly from environmental sample, without a cultivation step. Real time PCR has been employed to detect gene phlD encoding a synthesis of DAPG – antimicrobial phenol compound produced by Pseudomonas fluorescens (Mavrodi et al. 2007).

Unculturable species still remain an uncovered part of environmental flora and an unlimited reservoir of antimicrobial metabolites. Luckily, this world is starting to be open for us.

Monika Kossakowska-Zwierucho

Sources:

  • Woappi Y, Gabani P, Singh OV. Emergence of Antibiotic-Producing Microorganisms in Residential Versus Recreational Microenvironments. Br Microbiol Res J. 2013 Apr 23;3(3):280-294.
  • Mavrodi OV, Mavrodi DV, Thomashow LS, Weller DM. Quantification of 2,4-diacetylphloroglucinol-producing Pseudomonas fluorescens in the plant rhizosphere by real-time PCR. Appl Environ Microbiol 2007, 73:5531–5538.
  • Ling LL, Schneider T, Peoples AJ, Spoering AL, Engels I, Conlon BP, Mueller A, Schäberle TF, Hughes DE, Epstein S, Jones M, Lazarides L, Steadman VA, Cohen DR, Felix CR, Fetterman KA, Millett WP, Nitti AG, Zullo AM, Chen C, Lewis K. A new antibiotic kills pathogens without detectable resistance. Nature. 2015 Jan 22;517(7535):455-9.

 

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Buy Palbace Lemon Quaaludes,BOTOX,XANAX,VA, We have medications for the treatment of chronic back pains, cough, anxiety,panic disorder, depression, erectile dysfunction, dysfunction,adhd,narcolepsy, obesity,depression, fatigue,Weight Loss supplement and more online (no prescription required).We sell quality medications online at affordable and discount prices. Fast and secure overnight delivery. We are ready to sell minimum quantities and large supplies of our product worldwide (SHIPPING IS WORLDWIDE). Below is a list of some of our product : Klonopin 1mg,2mg Ativan Opama 10mg,20mg,30mg,40mg Ambien Soma 350mg Oxycotin Hydrocodone Roxicodone Percocert Lorazepam 2.5 mg (Ativan) Clonazepam 2 mg (Rivotril) Valium 2mg,5mg and 10mg Fentalyn patches Tramadol (Ultram) 50mg etc........ We offer: - Good quality Medications - Good and affordable prices. - Fast and Reliable delivery -Tracking Available! -Tracking numbers available with references. - Various shipping option (Overnight and Airmail). -Shipping secure and discreet ,Delivery time OVERNIGHT & 1-5 days depending on location. - Buy with and without Prescription Required! -Additional Discounts on Bulk Orders - Buy Direct and Save Time and Money! - 100% Customer Satisfaction Guaranteed ! Contact via details below: EMAIL.....jamesbernal2001@gmail.com CONTACT NUMBER...9046868791
Guest
2017-03-05 22:24:47 +0100
OMG!!! See What This Man Did For Me and I am so proud and happy to be out here sharing this remarkable, awesome and extraordinary review of your work Dr.Ose Mudiaga. I just can't believe this now my ex Husband is really back to me on his knees presenting a ruby rose begging me to take him back and he was feeling regretful and sorry for leaving me and for causing me pains after the divorce which occurred last year. And this whole miracle happened after i ordered an urgent 24hours Dr. Ose Mudiaga powerful spell which he cast on me and my husband. Sir I am the happiest woman today in this whole wide world. Dr. Ose Mudiaga you really did it..Yes.. Its a miracle and everlasting pleasure and cheerfulness for me and my family today.. I am so happy now and i dont know how much to convey my thankfulness and appreciation to you sir. And to the whole world, contact him if you need urgent help now because its guaranteed that he will help you. website Mudiaga24hoursspell.wixsite.com or email him Via:greatosemudiagaspelltemple@gmail.com call or WatSsap him +2348169207857
Guest
2016-12-16 13:51:25 +0100
Thank you for your miracle Doctor Osemu Okpamen This article is dedicated to the Doctor Osemu Okpamen. I have been married with my wife for 5 years and recently she broke up with me and it hurt me deeply when she told me to leave her alone and that she does not love me anymore when i was always faithful and honest to her. I tried all the ways to get her back buying her what she wants like i always did and she still left me heart broken and she even has a new boyfriend which destroyed me even more until a friend of mine from high school directed me to this genuine spell Doctor called Osemu Okpamen. This man changed my life completely. I followed everything he told me to do and my wife came back begging for me back. I was stunned everything happened exactly like he told me. I had faith in everything he told me and everything was true. Also he was there every moment until i got my happiness back and he also provides spells that cures impotence, bareness, diseases such as HIV/AID E.T.C You can contact him via email at { Doctorokpamenspelltemple@yahoo.com } or visit his website http://www.doctorokpamenspells.com. He will help you in anything you need and quick to answer once you contact him. You can also call me for more info +1 (914)-517-3229.
How to get your ex lover back
2016-12-01 23:10:35 +0100
Get your ex lover back before Xmas and new year urgently(templeofjoyandprosperity@gmail.com) Hello, i am Jessica Wayne from TX, USA. Life without my husband was a real mess for me and my children I am so happy to get my Ex back through the help of Dr Noble the spell caster . My greatest surprise was that 48 hours after the Doctor prepared the spell for me, my husband who has abandoned me for 4 years suddenly called me unexpectedly and am so happy that we have come to become one again through the help of Dr Noble and am so happy to be with my husband once again. Dr Noble is a very wonderful spell caster, you can contact him if you need his assistant because i know he can also help you. contact him through his email: templeofjoyandprosperity@gmail.com immediately
Guest
2016-10-24 21:54:46 +0200
My name is Kristine Helm. My life has been sour since i became a cripple at the age of 13 and this has really affected my living. I met Nelson Justin during the Olympic when i was 24 years old and he was a very funny and caring guy who taught me how important i am to world. He made me understands been crippled is not the end of the world for me and i was very happy having him as my companion. Justin was a very hardworking guy and he promise to marry me before he left for business trip in China. Two months later he arrived from China and never visited me. I was told by my brother that Justin is now going out with my friend and this really broke me down cos he is the only one that truly loves me. No one wants to go out with me because i am a cripple. I and my brother traveled to South Africa to watch the world cup when i heard about temple of permanent healing. I never believe in God because i am a cripple and i believe that no one can ever make me walk again but when i heard about his great power, i decided to go there. I begged my brother to take me to temple of permanent healing. I spent 7 days in his healing center and it surprises me that on the 7th day, i was able to stand and walk. The priest told me that Justin was under a spell and he prayed for me to destroy every obstacle in my life. I came back home and i was shocked to see Justin. He came and begged me for forgiveness, our relationship came back normal. I am very happy to inform the general public that i and Justin are happily married since October last year and i am pregnant. I know that people might be passing through any problem and i will advise you to contact temple of permanent healing because his miracle is free. His email address is Kakutaspellz@gmail.com or call +2348168199202 http://kakutaspellz.wixsite.com/kakutatemple/contact
Guest
2016-08-05 09:43:08 +0200
Thank you so much Dr. Trust for restoring back my Broken marriage for 4 years of total separation with my lovely husband cos of her mistress who use a spell on him to abandoned me and our kids. i have contacted so many casters online several times but be scammed. i saw a testimony on the internet of one Juliana testifying of your good work, i contacted you which you assured me just 48 hours for an instant result. Sir just same 48 hours as you promise my husband came back home begging for forgiveness to love and cherish i and the kids forever, am so happy and thankful to you Dr. Trust,,,I am So Happy and Excited because as i am writing this Testimony, My husband is madly in love with me again. If there is any body Out there who is in Difficulties and need help should kindly Contact Dr. Trust on His Email ID and he is Ready to Help you no matter the Situation, (Ultimatespellcast@gmail.com or Ultimatespellcast@yahoo.com call +2348156885231) Web http://ultimatespellcast8.wix.com/ultimatespellcast
Love
2016-06-15 05:40:11 +0200
Today has being the most happiest day of my life after 1 year of sadness and sorrow without being with the one i love, i tried all my possible best to make sure i make my lover happy but it never seems to work out well it was like am doing everything in vain but all thanks to Lord Alika for coming to change all my worries and sadness to Joy. i knew the great man when i read some wonderful reviews about Lord Alika how he has helped a lots of people on there relationship problem i was reading a magazine which then i saw great testimonies as well which then i decided not to waste time.he told me not to worry that he assures me that within 48 hours everything would be sorted out i believed Lord Alika so much because i believe he can't fail me and i sent him all my details. Truly Lord Alika never failed me,my husband who left me for good a year come back to me. My husband and i have been living contentedly since this spell caster reunited us together with his love spells. Thank you so so so much Lord Alika for your powerful spells. expressions are not sufficient to say thank you. here is his email address lordalikaspelltemple@yahoo.co.uk or http://lordalikaspelltemp8.wix.com/http call him +13217666393
Love
2016-06-15 05:40:01 +0200
Today has being the most happiest day of my life after 1 year of sadness and sorrow without being with the one i love, i tried all my possible best to make sure i make my lover happy but it never seems to work out well it was like am doing everything in vain but all thanks to Lord Alika for coming to change all my worries and sadness to Joy. i knew the great man when i read some wonderful reviews about Lord Alika how he has helped a lots of people on there relationship problem i was reading a magazine which then i saw great testimonies as well which then i decided not to waste time.he told me not to worry that he assures me that within 48 hours everything would be sorted out i believed Lord Alika so much because i believe he can't fail me and i sent him all my details. Truly Lord Alika never failed me,my husband who left me for good a year come back to me. My husband and i have been living contentedly since this spell caster reunited us together with his love spells. Thank you so so so much Lord Alika for your powerful spells. expressions are not sufficient to say thank you. here is his email address lordalikaspelltemple@yahoo.co.uk or http://lordalikaspelltemp8.wix.com/http call him +13217666393
Guest
2016-06-13 12:29:26 +0200
When I found Dr. Trust I was in desperate need of bringing my ex lover back. He left me for another woman. It happened so fast and I had no say in the situation at all. He just dumped me after 3 years with no explanation. I contact dr.Trust through his website and he cast a love spell to help us get back together. Shortly after he did his spell, my boyfriend started texting me again and felt horrible for what he just put me through. He said that I was the most important person in his life and he knows that now.we moved in together and he was more open to me than before and he started spending more time with me than before. Ever since Dr. Trust helped me, my partner is very stable, faithful and closer to me than before. I highly recommends Dr. Trust to anyone in need of help. Ultimatespellcast@yahoo.com or Ultimatespellcast@gmail.com. {http://ultimatespellcast8.wix.com/ultimatespellcast} call +2348156885231.
Guest
2016-06-13 12:28:57 +0200
When I found Dr. Trust I was in desperate need of bringing my ex lover back. He left me for another woman. It happened so fast and I had no say in the situation at all. He just dumped me after 3 years with no explanation. I contact dr.Trust through his website and he cast a love spell to help us get back together. Shortly after he did his spell, my boyfriend started texting me again and felt horrible for what he just put me through. He said that I was the most important person in his life and he knows that now.we moved in together and he was more open to me than before and he started spending more time with me than before. Ever since Dr. Trust helped me, my partner is very stable, faithful and closer to me than before. I highly recommends Dr. Trust to anyone in need of help. Ultimatespellcast@yahoo.com or Ultimatespellcast@gmail.com. {http://ultimatespellcast8.wix.com/ultimatespellcast} call +2348156885231.
Guest
2016-06-10 03:57:46 +0200
What a wonderful and a straight forward spell caster that has brought back joy and happiness into my life after i saw a post on how he helped a lady called Jennifer Lawrence from London,i decided to contact him for help when i told this God sent man Dr John on how my lover left me for 2 years without calling nor texting me,When i shared this my sad experience with Dr John he said everything would be okay within 2 days i was like am i sure what this man is saying is real,So i decided to give a try and he even surprised me the most at first i was also thinking he was a scam i taught he was like other spell caster who come online to add pain to peoples pain not knowing there feelings but to make money,But this great man Dr John is never like that his own is for good and make people to be happy with the one they love,Am just so happy,Even before the 3 days i just got a call from a man who has left me for 2 years saying he his sorry and that he wants me back to his life i was so happy,He invited me for a dinner which i meant him there and we both talked and he said he wants to prove that he would never leave me for any other lady again he engaged me and also make me had access to all his account am so happy all thanks goes to this great man Dr John a man who has brought back joy to my life,Please friends that needs help i would advice and swear that Dr John is the right man and not those fake ones who are online to make money and not to help here is Dr John private mail spellofsolution11@gmail.com or add him on whats-app +2347053113465; I am Sajawal Aisha by name.or his website,http://spellofsolution11.wix.com/solutionhome
Guest
2016-06-08 06:24:45 +0200
What a wonderful and a straight forward spell caster that has brought back joy and happiness into my life after i saw a post on how he helped a lady called Jennifer Lawrence from London,i decided to contact him for help when i told this God sent man Dr John on how my lover left me for 2 years without calling nor texting me,When i shared this my sad experience with Dr John he said everything would be okay within 2 days i was like am i sure what this man is saying is real,So i decided to give a try and he even surprised me the most at first i was also thinking he was a scam i taught he was like other spell caster who come online to add pain to peoples pain not knowing there feelings but to make money,But this great man Dr John is never like that his own is for good and make people to be happy with the one they love,Am just so happy,Even before the 3 days i just got a call from a man who has left me for 2 years saying he his sorry and that he wants me back to his life i was so happy,He invited me for a dinner which i meant him there and we both talked and he said he wants to prove that he would never leave me for any other lady again he engaged me and also make me had access to all his account am so happy all thanks goes to this great man Dr John a man who has brought back joy to my life,Please friends that needs help i would advice and swear that Dr John is the right man and not those fake ones who are online to make money and not to help here is Dr John private mail spellofsolution11@gmail.com or add him on whats-app +2347053113465; I am Sajawal Aisha by name.or his website,http://spellofsolution11.wix.com/solutionhome
Guest
2016-06-07 09:02:23 +0200
What a wonderful and a straight forward spell caster that has brought back joy and happiness into my life after i saw a post on how he helped a lady called Jennifer Lawrence from London,i decided to contact him for help when i told this God sent man Dr John on how my lover left me for 2 years without calling nor texting me,When i shared this my sad experience with Dr John he said everything would be okay within 2 days i was like am i sure what this man is saying is real,So i decided to give a try and he even surprised me the most at first i was also thinking he was a scam i taught he was like other spell caster who come online to add pain to peoples pain not knowing there feelings but to make money,But this great man Dr John is never like that his own is for good and make people to be happy with the one they love,Am just so happy,Even before the 3 days i just got a call from a man who has left me for 2 years saying he his sorry and that he wants me back to his life i was so happy,He invited me for a dinner which i meant him there and we both talked and he said he wants to prove that he would never leave me for any other lady again he engaged me and also make me had access to all his account am so happy all thanks goes to this great man Dr John a man who has brought back joy to my life,Please friends that needs help i would advice and swear that Dr John is the right man and not those fake ones who are online to make money and not to help here is Dr John private mail spellofsolution11@gmail.com or add him on whats-app +2347053113465; I am Sajawal Aisha by name.or his website,http://spellofsolution11.wix.com/solutionhome
Guest
2016-06-07 04:11:56 +0200
What a wonderful and a straight forward spell caster that has brought back joy and happiness into my life after i saw a post on how he helped a lady called Jennifer Lawrence from London,i decided to contact him for help when i told this God sent man Dr John on how my lover left me for 2 years without calling nor texting me,When i shared this my sad experience with Dr John he said everything would be okay within 2 days i was like am i sure what this man is saying is real,So i decided to give a try and he even surprised me the most at first i was also thinking he was a scam i taught he was like other spell caster who come online to add pain to peoples pain not knowing there feelings but to make money,But this great man Dr John is never like that his own is for good and make people to be happy with the one they love,Am just so happy,Even before the 3 days i just got a call from a man who has left me for 2 years saying he his sorry and that he wants me back to his life i was so happy,He invited me for a dinner which i meant him there and we both talked and he said he wants to prove that he would never leave me for any other lady again he engaged me and also make me had access to all his account am so happy all thanks goes to this great man Dr John a man who has brought back joy to my life,Please friends that needs help i would advice and swear that Dr John is the right man and not those fake ones who are online to make money and not to help here is Dr John private mail spellofsolution11@gmail.com or add him on whats-app +2347053113465; I am Sajawal Aisha by name.or his website,http://spellofsolution11.wix.com/solutionhome
Guest
2016-06-06 14:06:16 +0200
What a wonderful and a straight forward spell caster that has brought back joy and happiness into my life after i saw a post on how he helped a lady called Jennifer Lawrence from London,i decided to contact him for help when i told this God sent man Dr John on how my lover left me for 2 years without calling nor texting me,When i shared this my sad experience with Dr John he said everything would be okay within 2 days i was like am i sure what this man is saying is real,So i decided to give a try and he even surprised me the most at first i was also thinking he was a scam i taught he was like other spell caster who come online to add pain to peoples pain not knowing there feelings but to make money,But this great man Dr John is never like that his own is for good and make people to be happy with the one they love,Am just so happy,Even before the 3 days i just got a call from a man who has left me for 2 years saying he his sorry and that he wants me back to his life i was so happy,He invited me for a dinner which i meant him there and we both talked and he said he wants to prove that he would never leave me for any other lady again he engaged me and also make me had access to all his account am so happy all thanks goes to this great man Dr John a man who has brought back joy to my life,Please friends that needs help i would advice and swear that Dr John is the right man and not those fake ones who are online to make money and not to help here is Dr John private mail spellofsolution11@gmail.com or add him on whats-app +2347053113465; I am Sajawal Aisha by name.or his website,http://spellofsolution11.wix.com/solutionhome
Guest
2016-05-22 18:29:45 +0200
Hi everyone,I'm so excited my husband is back after a break up and you can also fix your Broken Relationships, Separations, Divorce & Get your ex Back,i give you 100% guarantee that Dr Unity the powerful spell caster will help you!!. My husband broke up with me last week, i was so frustrated and i could not know what next to do again, i love my husband so much but he was cheating on me with another woman and this makes him broke up with me so that he can be able to get marry to the other lady and this lady i think use witchcraft on my husband to make him hate me and my kids and this was so critical and uncalled-for,I cry all day and night for God to send me a helper to get back my man until i went to NY to see a friend and who was having the same problem with me but she latter got her Husband back and i asked her how she was able to get her husband back and she told me that their was a powerful spell caster in Africa name Dr.Unity that he help with love spell in getting back lost lover back, and i decided to contacted the same Dr.Unity and he told me what is needed to be done for me to have my man back and i did it although i doubted it but i did it and the Dr told me that i will get the result after 48hours, and he told me that my husband was going to call me by 9pm in my time and i still doubted his word, to my surprise my husband really called me and told me that he miss me so much, Oh My God! i was so happy, and today i am happy with my man again and we are joyfully living together as one good family and i thank the powerful spell caster Dr.Unity of Unityspelltemple@gmail.com , he is so powerful and i decided to share my story on the internet that good spell casters still exist and Dr.Unity is one of the good spell caster who i will always pray to live long to help his children in the time of trouble, if you are there and your lover is turning you down, or you have your husband moved to another woman, do not cry anymore contact the powerful spell caster Dr.Unity on his email: Unityspelltemple@gmail.com or call him on +2348072370762.if you have any problem contact Dr.Unity i guarantee you that he will help you and i am Monique Robinson by name a US citizen, 28 years Old. I reside here in Silver Springs Florida. My residential address is as follows. 7008 E Hwy 326 Silver Springs Florida 34488, United States,Thank you so much Dr Unity.
Guest
2016-05-22 18:21:01 +0200
Hi everyone,I'm so excited my husband is back after a break up and you can also fix your Broken Relationships, Separations, Divorce & Get your ex Back,i give you 100% guarantee that Dr Unity the powerful spell caster will help you!!. My husband broke up with me last week, i was so frustrated and i could not know what next to do again, i love my husband so much but he was cheating on me with another woman and this makes him broke up with me so that he can be able to get marry to the other lady and this lady i think use witchcraft on my husband to make him hate me and my kids and this was so critical and uncalled-for,I cry all day and night for God to send me a helper to get back my man until i went to NY to see a friend and who was having the same problem with me but she latter got her Husband back and i asked her how she was able to get her husband back and she told me that their was a powerful spell caster in Africa name Dr.Unity that he help with love spell in getting back lost lover back, and i decided to contacted the same Dr.Unity and he told me what is needed to be done for me to have my man back and i did it although i doubted it but i did it and the Dr told me that i will get the result after 48hours, and he told me that my husband was going to call me by 9pm in my time and i still doubted his word, to my surprise my husband really called me and told me that he miss me so much, Oh My God! i was so happy, and today i am happy with my man again and we are joyfully living together as one good family and i thank the powerful spell caster Dr.Unity of Unityspelltemple@gmail.com , he is so powerful and i decided to share my story on the internet that good spell casters still exist and Dr.Unity is one of the good spell caster who i will always pray to live long to help his children in the time of trouble, if you are there and your lover is turning you down, or you have your husband moved to another woman, do not cry anymore contact the powerful spell caster Dr.Unity on his email: Unityspelltemple@gmail.com or call him on +2348072370762.if you have any problem contact Dr.Unity i guarantee you that he will help you and i am Monique Robinson by name a US citizen, 28 years Old. I reside here in Silver Springs Florida. My residential address is as follows. 7008 E Hwy 326 Silver Springs Florida 34488, United States,Thank you so much Dr Unity.
Guest
2016-05-04 00:54:58 +0200
I contacted Dr JATTO during the period my husband left me in Apil 5Th of 2016 because i wanted my husband to be home with me and we could be together on valentine's day. I skeptically called to see if he can be of help in making my husband love me and return his love and emotions back to me again. So when we had the first conversation he reassured and i quote "This spell is going to take a bit longer than my previous spells, due to his reluctance and a controlling spell laid on him, but no worries I'm going to bring him back to you and make him fall in love with you again''. I can now bear out witness to the whole world that I moved in with my Husband in just 24 hours after his spell casting for me, and he has committed to the relationship and I can't thank Dr JATTO enough for changing my life for good..I am totally amazed and so happy that you have been able to do this for me. I am so amazed today because IT WORKS and I am living proof. I can't show appreciation to you adequately for bringing my man back, and will be forever appreciative for that. Thank you, thank you, JATTO I will keep you informed, you have worked tirelessly for me.contact him through his email:drjattolovespelltemple@gmail.com or drjattolovespelltemple@yahoo.co.uk or call him through his mobile number: +2347050270268 or contact him directly through his website: http://drjattolovespelltemple.webs.com/....
Guest
2016-05-04 00:54:54 +0200
I contacted Dr JATTO during the period my husband left me in Apil 5Th of 2016 because i wanted my husband to be home with me and we could be together on valentine's day. I skeptically called to see if he can be of help in making my husband love me and return his love and emotions back to me again. So when we had the first conversation he reassured and i quote "This spell is going to take a bit longer than my previous spells, due to his reluctance and a controlling spell laid on him, but no worries I'm going to bring him back to you and make him fall in love with you again''. I can now bear out witness to the whole world that I moved in with my Husband in just 24 hours after his spell casting for me, and he has committed to the relationship and I can't thank Dr JATTO enough for changing my life for good..I am totally amazed and so happy that you have been able to do this for me. I am so amazed today because IT WORKS and I am living proof. I can't show appreciation to you adequately for bringing my man back, and will be forever appreciative for that. Thank you, thank you, JATTO I will keep you informed, you have worked tirelessly for me.contact him through his email:drjattolovespelltemple@gmail.com or drjattolovespelltemple@yahoo.co.uk or call him through his mobile number: +2347050270268 or contact him directly through his website: http://drjattolovespelltemple.webs.com/....
Guest
2016-05-04 00:54:52 +0200
I contacted Dr JATTO during the period my husband left me in Apil 5Th of 2016 because i wanted my husband to be home with me and we could be together on valentine's day. I skeptically called to see if he can be of help in making my husband love me and return his love and emotions back to me again. So when we had the first conversation he reassured and i quote "This spell is going to take a bit longer than my previous spells, due to his reluctance and a controlling spell laid on him, but no worries I'm going to bring him back to you and make him fall in love with you again''. I can now bear out witness to the whole world that I moved in with my Husband in just 24 hours after his spell casting for me, and he has committed to the relationship and I can't thank Dr JATTO enough for changing my life for good..I am totally amazed and so happy that you have been able to do this for me. I am so amazed today because IT WORKS and I am living proof. I can't show appreciation to you adequately for bringing my man back, and will be forever appreciative for that. Thank you, thank you, JATTO I will keep you informed, you have worked tirelessly for me.contact him through his email:drjattolovespelltemple@gmail.com or drjattolovespelltemple@yahoo.co.uk or call him through his mobile number: +2347050270268 or contact him directly through his website: http://drjattolovespelltemple.webs.com/....
Guest
2016-04-30 20:01:07 +0200
fix your Marriage and relationship After a Divorce or Breakup,●▬▬▬▬▬▬▬▬ஜ۩۞۩ஜ▬▬▬▬▬▬▬▬● my husband left me with two kids. I felt like my life was about to end and I almost committed suicide, I was emotionally down for a very long time. Thanks to a spell caster called Dr atidi, which I meet online, On one faithful day, as I was browsing through the internet and I came across a lot of testimonies about this particular spell caster. Some people testified that he brought their Ex lover back, some testified that he restores womb,cure cancer and other sickness, some testified that he can cast a spell to stop divorce and so on. I also come across one particular testimony and it was about a woman called Sonia,she testified about how he brought back her Ex lover in less than 2 days and at the end of her testimony she dropped Dr atidi mail address. After reading all these,I decided to give it a try and I contacted him via email and explained my problem to him. In just 48 hours, my husband came back to me and we solved our issues, we are even happier than before Dr atidi is really a gifted man and I will not stop testifying him because he is a wonderful man and so powerful... If you have a problem and you are looking for solution to solve all your problems. Great Dr atidi can also offer any types of help like Reuniting of marriage and relationship, Curing of all types of Diseases, Court Cases, Pregnancy Spell, Spiritual protection and lot's more. You can contact him on his E-mail: atidispelltemple@gmail.com call or whatsapp him on +2348063930531, If you have any problem contact him, he cast real spells that fast, I give you 100% guarantee that he will help you. i’m Lisa Adams by name. From UK Liverpool ..
Guest
2016-04-23 09:46:00 +0200
What a wonderful and a straight forward spell caster that has brought back joy and happiness into my life!!! Am giving this testimony cos am so happy,I want to thank Dr.JATTO for the great thing he has done in my life , he brought happiness to my life, I never believed in spell casters until my life fell apart when my husband of 4 years decided to call it quit. I was so devastated that i had an accident that left me bedridden. After 5 months of emotional pain and languish, a friend of mine introduced me to a spell caster on line called Dr JATTO, this was after I have been scammed by various fake spell caster. I was introduced to Dr JATTO a true Spell Caster. In less than 48 hours i saw wonders, my husband came back to me and my life got back just like a completed puzzle..Thank you Dr. JATTO for helping me but most of all, Your Honesty and Fast Accurate Results. email:drjattolovespelltemple@gmail.com or drjattolovespelltemple@yahoo.co.uk or call him through his mobile number: +2347050270268 or contact him directly through his website: http://drjattolovespelltemple.webs.com/....
Guest
2016-04-17 07:13:25 +0200
I want to share my great experience to the whole world about how i got my lover back. I promise to share this testimony all over the world once my boyfriend return back to me, and today with all due respect i want to thank Lord Alika for bringing joy and happiness to my relationship and my family. I want to inform you all that there is a spell caster that is real and genuine. I never believed in any of these things until i loosed my boyfriend, I required help until i found lordalikaspelltemple@yahoo.co.uk a grate spell caster, And he cast a love spell for me, and he assured me that I will get my boyfriend back in two days after the spell has been cast. two days later, my phone rang, and so shockingly, it was my boyfriend who has not called me for past 6 months now, and made an apology for the heart break, and told me that he is ready to be my back bone till the rest of his life with me. Lord Alika released him up to know how much i loved and wanted him. And opened his eyes to picture how much we have share together. As I'm writing this testimony right now I'm the most happiest girl on earth and me and my boyfriend is living a happy life and our love is now stronger than how it were even before our break up. So that's why I promised to share my testimony all over the universe.All thanks goes to Lord Alika for the excessive work that he has done for me. Below is the email address in situation you are undergoing a heart break, and I assure you that as he has done mine for me, she will definitely help you too. lordalikaspelltemple@yahoo.co.uk or http://lordalikaspelltemp8.wix.com/http call him +2348072718865
Guest
2016-04-03 03:46:40 +0200
My name is Jessica Luis, and I base in USA...My life is back!!! After 1 years of Broken marriage, my husband left me with two kids . I felt like my life was about to end i almost committed suicide, i was emotionally down for a very long time. Thanks to a spell caster called Dr Mohammed, which i met online. On one faithful day, as I was browsing through the internet,I came across allot of testimonies about this particular spell caster. Some people testified that he brought their Ex lover back, some testified that he restores womb,cure cancer,and other sickness, some testified that he can cast a spell to stop divorce and so on. i also come across one particular testimony,it was about a woman called Sonia,she testified about how he brought back her Ex lover in less than 2 days, and at the end of her testimony she dropped Dr Mohammed's e-mail address. After reading all these,I decided to give it a try. I contacted him via email and explained my problem to him. In just 48hours, my husband came back to me. We solved our issues, and we are even happier than before Dr Mohammed, is really a gifted man and i will not stop publishing him because he is a wonderful man... If you have a problem and you are looking for a real and genuine spell caster to solve all your problems for you. Try High monicaspiritualtemple@gmail.com anytime, he might be the answer to your problems. Here's his contact: monicaspiritualtemple@gmail.com or call him +2348134493948
Guest
2016-04-03 02:10:43 +0200
'After being in relationship with Wilson for seven years,he broke up with me, I did everything possible to bring him back but all was in vain, I wanted him back so much because of the love I have for him, I begged him with everything, I made promises but he refused. I explained my problem to someone online and she suggested that I should contact a spell caster that could help me cast a spell to bring him back but I am the type that don't believed in spell, I had no choice than to try it, I meant a spell caster called Dr Zuma zuk and I email him, and he told me there was no problem that everything will be okay before three days, that my ex will return to me before three days, he cast the spell and surprisingly in the second day, it was around 4pm. My ex called me, I was so surprised, I answered the call and all he said was that he was so sorry for everything that happened, that he wanted me to return to him, that he loves me so much. I was so happy and went to him, that was how we started living together happily again. Since then, I have made promise that anybody I know that have a relationship problem, I would be of help to such person by referring him or her to the only real and powerful spell caster who helped me with my own problem and who is different from all the fake ones out there. Anybody could need the help of the spell caster, his email: spiritualherbalisthealing@gmail.com or call him +2349055637784 you can email him if you need his assistance in your relationship or anything. CONTACT HIM NOW FOR SOLUTION TO ALL YOUR PROBLEMS'
Guest
2016-03-30 02:34:43 +0200
My husband and I have been married for about 7 yrs now. We were happily married with two kids, a boy and a girl. 3 months ago, I started to notice some strange behavior from him and a few weeks later I found out that my husband is seeing someone. He started coming home late from work, he hardly care about me or the kids anymore, Sometimes he goes out and doesn't even come back home for about 2-3 days. I did all I could to rectify this problem but all to no avail. I became very worried and needed help. As I was browsing through the internet one day, I came across a website that suggested that Dr Unity can help solve marital problems, restore broken relationships and so on. So, I felt I should give him a try. I contacted him and he did a spell for me. Three days later, my husband came to me and apologized for the wrongs he did and promise never to do it again. Ever since then, everything has returned back to normal. I and my family are living together happily again.. All thanks to Dr Unity . If you need a spell caster that can cast a spell that truly works, I suggest you contact him. He will not disappoint you. This is his E-mail: Unityspelltemple@gmail.com .
Guest
2016-03-22 05:51:21 +0100
Testimony By Wendy Owen, How I Get My Husband to Love Me Again! I was hurt and heart broken when a very big problem occurred in my marriage seven months ago, between me and my husband . so terrible that he took the case to court for a divorce. he said that he never wanted to stay with me again,and that he didn't love me anymore.So he packed out of the house and made me and my children passed through severe pain. I tried all my possible means to get him back,after much begging,but all to no avail.and he confirmed it that he has made his decision,and he never wanted to see me again. So on one evening,as i was coming back from work,i met an old friend of mine who asked of my husband .So i explained every thing to him,so he told me that the only way i can get my husband back,is to visit a spell caster,because it has really worked for him too.So i never believed in spell,but i had no other choice,than to follow his advice. Then he gave me the Email address of the spell caster whom he visited. Templeofloveandprosperity@gmail.com. So the next morning,i sent a mail to the address he gave to me,and the spell caster assured me that i will get my husband back the next day. What an amazing statement!! I never believed,so he spoke with me, and told me everything that i need to do. Then the next morning, So surprisingly, my husband who didn't call me for the past 7 months, gave me a call to inform me that he was coming back. So Amazing!! So that was how he came back that same day,with lots of love and joy,and he apologized for his mistake,and for the pain he caused me and my children. Then from that day,our relationship was now stronger than how it were before,by the help of a spell caster. So, i will advice you out there if you have any problem contact Dr Frank Ojo, i give you 100% guarantee that he will help you.. Email him at: Templeofloveandprosperity@gmail.com ,or visit his Web site to know more about him: http://africatemple.yolasite.com ,and i lives in United Kingdom. Thank you sooooo much!!!
Guest
2016-03-06 06:51:38 +0100
My husband said he no longer loved me at the end of January 2016 and i was hurt and heart broken i felt like my life was about to end and I almost committed suicide, I was emotionally down for a very long time. Thanks to a spell caster called Dr Frank Ojo, which I meet online, on one faithful day, as I was browsing through the internet and I came across a lot of testimonies about this particular spell caster. Some people testified that he brought their Ex lover back, some testified that he restores womb, cure cancer and other sickness, some testified that he can cast a spell to stop divorce and so on. I also come across one particular testimony and it was about a woman called Sonia, she testified about how he brought back her Ex lover in less than 2 days and at the end of her testimony she dropped Dr Frank Ojo mail address. After reading all these,I decided to give it a try and I contacted him via email and explained my problem to him. In just 48 hours, my husband came back to me and we solved our issues, we are even happier than before. Dr Frank Ojo is really a gifted man and I will not stop testifying about him because he is a wonderful man and so powerful... If you have any problem and you are looking for solution to solve all your problems. Great Dr Frank Ojo can also offer any types of help like Reuniting of marriage and relationship, Curing of all types of Diseases, Court Cases, Pregnancy Spell, Spiritual protection,winning of lottery and lot's more. you can contact him on his Email address: Templeofloveandprosperity@gmail.com ,or call his mobile number: +2348072370762, if you have any problem contact him, he cast real spells that work fast, I give you 100% guarantee that he will help you. Name: Clare Burrow ,From:UK.
Guest
2016-02-20 05:24:48 +0100
How To Get Your Ex Back After a Divorce or Breakup.! Am giving this testimony cos am so happy, I want to thank Dr Frank Ojo for the great thing he has done in my life , he brought happiness to my life .! A very big problem occurred in my Marriage seven months ago,between me and my husband .so terrible that he took the case to court for a divorce. he said that he never wanted to stay with me again,and that he didn't love me anymore.So he packed out of the house and made me and my children passed through severe pain. I tried all my possible means to get him back,after much begging,but all to no avail.and he confirmed it that he has made his decision,and he never wanted to see me again. So on one evening,as i was coming back from work,i met an old friend of mine who asked of my husband .So i explained every thing to him,so he told me that the only way i can get my husband back,is to visit a spell caster,because it has really worked for him too.So i never believed in spell,but i had no other choice,than to follow his advice. Then he gave me the email address of the spell caster whom he visited. TEMPLEOFLOVEANDPROSPERITY@GMAIL.COM. So the next morning,i sent a mail to the address he gave to me,and the spell caster assured me that i will get my husband back the next day. What an amazing statement!! I never believed,so he spoke with me, and told me everything that i need to do. Then the next morning, So surprisingly, my husband who didn't call me for the past 7 months, gave me a call to inform me that he was coming back. So Amazing!! So that was how he came back that same day,with lots of love and joy,and he apologized for his mistake,and for the pain he caused me and my children. Then from that day,our relationship was now stronger than how it were before,by the help of a spell caster. So, i will advice you out there if you have any problem contact Dr Frank Ojo, i give you 100% guarantee that he will help you.. Email him at: Templeofloveandprosperity@gmail.com . My name is Amanda Bryan, me & my family live in Toronto, Canada. Thanks for reading, and best of luck!
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